05-200-1A MSC NutriStem XF serum-free medium

Product Name:05-200-1A MSC NutriStem XF serum-free medium

Product Code: 05-200-1A

Product specifications: 500mL/bottle (liquid, containing phenol red); No serum, no heterologous animal components, limited chemical composition; 0.22 μ m filtration for sterilization, endotoxin<0.5EU/mL, sterile and free of mycoplasma.

Product Overview: 05-200-1A MSC NutriStem ® XF is a serum-free culture medium for MSCs under Sartorius (formerly Bioind/BI), specifically designed for efficient expansion and maintenance of mesenchymal stem cells (hMSCs) from multiple sources such as human bone marrow, adipose tissue, and umbilical cord,Serum free, Xeno free, 100% chemical definition of ingredientsIt supports primary isolation, long-term passaging (≥ 15 generations), stable three-way differentiation, and clinical grade extracellular vesicle preparation, and is the gold standard culture medium for stem cell therapy, regenerative medicine, and drug application. Huayi Si Chuang Biotechnology is a legitimate authorized agent of Sartorius, with sufficient stock of 05-200-1A and transparent NutriStem prices. We provide complete COA, DMF documents and technical support.

Brand Introduction

Sartorius is a global leader in life sciences and biopharmaceuticals, with its subsidiary Bioind (BI) NutriStem ® The series of culture media have been certified by cGMP, ISO 9001, and ISO 13485, and have a US FDA DMF registration number (29469). They are widely used in phase II/III clinical trials worldwide and are known for their batch stability, high safety, and efficient amplification. They solve pain points such as serum contamination, large inter batch differences, and dry decay, helping stem cell drugs to be successfully declared.

Product parameters

Item number: 05-200-1A (basic culture medium, containing phenol red); Supporting additives: 05-201-1U (3mL)/05-201-106 (0.6mL)

Capacity: 500mL/bottle of basic culture medium

Applicable cells: hMSCs from all sources, including human bone marrow MSCs (BM-MSC), adipose derived MSCs (AT-MSC), umbilical cord MSCs (UC-MSC), dental pulp MSCs, etc

Core components: recombinant protein, vitamins, amino acids, minerals; No serum, no animal derived protein, no plant hydrolysates, no unknown components

Physical and chemical indicators: pH 7.2 ± 0.2, osmotic pressure 290 ± 15mOsm/kg

Aseptic standard: 0.22 μ m filtration sterilization, endotoxin<0.5EU/mL, sterile, free of mycoplasma, and free of viral contamination

Storage conditions: Store in the dark at 2-8 ℃, and the base liquid must not be frozen; Additives - stored at 20 ℃

Validity period: 12 months for unopened base solution; Mix additives and store at 4 ℃ for 14 days before use

Product features: limited chemical composition, no serum, no heterologous, containing phenol red, strong maintenance of dryness, efficient amplification, clinically compliant

core advantage

Serum free+heterologous+clinical grade safe elimination of serum and animal derived components, eliminating the risk of virus, mycoplasma, and heterologous protein contamination, in compliance with China's NMPA, US FDA, and EU EMA stem cell formulation standards, with full safety, FDA DMF filing (No. 29469), directly supporting clinical application.

Compared with serum containing medium, efficient amplification and dry strong maintenance increase the proliferation rate by 50% or more, and the cell activity is ≥ 95%; Continuously passaging for ≥ 15 generations to maintain stable * * CD73 ⁺/CD90 ⁺/CD105 ⁺ * * phenotype (purity>95%), with undiminished potential for osteogenic, adipogenic, and chondrogenic differentiation, and data repeatability>99%.

The ingredients are clear and stable between batches, with 100% chemical composition limitation and traceable source. The batch difference is less than 3%, eliminating serum batch fluctuations. The experimental results are highly stable and reproducible, suitable for high-precision scientific research and GMP production.

Extracellular vesicle friendly and widely applicable without serum background, with high purity, low impurities, and easy purification of extracellular vesicles, suitable for extracellular vesicle extraction, proteomics, and drug screening; Support 2D cultivation and 3D microcarrier suspension cultivation to meet scientific research and large-scale production needs.

Complete global clinical phase II/III extensive validation, cGMP production, CE certification, FDA DMF filing; Huaya Sichuang has stable stock and genuine product guarantee, provides complete declaration materials, and selects imported culture media.

Application scenarios

Basic research on stem cells: hMSC primary isolation, efficient amplification, stemness maintenance, and induction of three-way differentiation

Clinical grade cell preparation: research and development of stem cell therapy drugs, GMP scale production, IND application

Extracellular vesicle research and production: serum-free culture, high-purity extracellular vesicle extraction, extracellular vesicle drug development

Regenerative medicine: bone/cartilage/nerve repair, medical beauty anti-aging, immune regulation, tissue engineering

Drug screening: stem cell toxicity testing, efficacy evaluation, precision medicine model

Buying Guide

Confirm product combination: 05-200-1A is the basic culture medium and must be used in combination with 05-201-1U (3mL)/05-201-106 (0.6mL) additive. It cannot be cultured separately.

Differentiated version: 05-200-1A (including phenol red); The phenol free red version is 05-202-1, choose as needed.

Legitimate channels: Through the agent of Sartorius, Huayi Sichuang purchases, 05-200-1A in stock, genuine guarantee, and provides COA and DMF documents.

Storage and use: Base liquid should be kept away from light at 2-8 ℃, and freezing is strictly prohibited; Additive * * -20 ℃ * *; Mix before use and use within 14 days at 4 ℃.

Order Information

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