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Shanghai Yansheng Industrial Co., Ltd
Chicken pulmonary artery endothelial cells
NegotiableUpdate on 05/06
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Overview
The product being sold by Chicken Pulmonary Arterial Endothelial Cell Company: Phosphomannose Isomerase Antibody HMY-1 (Human Melanoma Cells), Rabbit Osteoclast Potassium/Sodium Hyperpolarization Activated Cyclic Nucleotide Gated Channel Protein 2 Antibody, Mouse Liver Kupffer Cell 17 β - Hydroxysteroid Dehydrogenase 12/17 β - HSD12 Antibody, Human Small Intestinal Mucosal Epithelial Cell Phosphorylated Complement Component 5 Receptor 1 Antibody
Product Details
Chicken pulmonary artery endothelial cells
Product attributes:
source of organization |
Product Specifications |
cell morphology |
Item Number |
Pulmonary artery tissue |
5×105cells/T25cell culture flask |
Endothelial cell like |
YS-01X7004 |
Cell Introduction:
Chicken pulmonary artery endothelium isolated from pulmonary artery tissue; The pulmonary artery, also known as the pulmonary artery trunk. In vertebrates that breathe air, the arteries that direct venous blood from the heart to the lungs. The pulmonary artery originates from the right ventricle and runs obliquely to the left, upper, and rear before the aorta. The cells are distributed in a single-layer polygonal cobblestone shape; This cell plays an important role in maintaining the dynamic balance inside and outside blood vessels, synthesizing and secreting cytokines and mediators, and maintaining the dynamic balance of coagulation and fibrinolysis. The endothelial cells of pulmonary arteries are distributed in a single-layer polygonal cobblestone shape. These cells play an important role in maintaining the dynamic balance inside and outside the blood vessels, synthesizing and secreting cytokines and mediators, and maintaining the dynamic balance of coagulation and fibrinolysis.
Cultivation conditions for primary cells:
1、 Culture of adherent cells (including semi adherent cells) in a static state
1. Cells should be thoroughly rinsed to remove the toxicity of digestive juices as much as possible;
2. When inoculating cells, the concentration should be slightly higher, at least 5 × 108 cells/L;
3. The culture medium can be Eagle (MEM Corning 10-010-CVR) or DMEM (Corning 10-013-CVR) for cultivation;
4. The concentration of fetal bovine serum is 10% -80%
5. It should be cultured in a 37 ℃ 5% CO2 incubator;
6. Try to minimize oscillation in the first 2 days to prevent newly adhered cells from shedding and floating;
7. When the cells are basically attached to the wall and gradually form a network, the primary cells should be replaced with fluid;
8. When using suspended cells from bone marrow or peripheral blood for one week of static culture, the cell suspension should be centrifuged at low speed and then replaced.
2、 Suspension cell culture
1. During primary culture, red blood cells should be removed as much as possible;
2. Short term cultivation can be carried out in RPMI1640 medium containing 10% calf serum;
3. Cell concentration can be within the range of 5-8 × 109/L for bottle separation experiments;
4. During long-term cultivation, growth factors should be added to lymphocytes, and a small amount of original patient serum should be added to leukemia cells to promote cell growth;
5. Cell fluid exchange usually requires a half volume exchange every 3 days;
6. Cell passage can only be carried out when cell proliferation accelerates and cell density is high.
Method Introduction:
The chicken pulmonary artery endothelium isolated in the company laboratory was used-It is prepared by combining collagenase digestion method with differential adhesion method and screening through endothelial cell specific culture medium, with a total cell count of approximately5×10?cells/Bottle.
Quality inspection:
Chicken pulmonary artery endothelial meridian isolated in the company laboratoryCD31Immunofluorescence identification, purity can reach90%Above, and not containingHIV-1TheHBVTheHCVMycoplasma, bacteria, yeast, fungi, etc.
Training Information:
Package conditionsPLL（0.1mg/ml）Gelatin（0.1%）
The culture medium containsFBSGrowth additivesPenicillinTheStreptomycinwait
Fluid change frequency per2-3Change the fluid once every day
Growth characteristics: Wall adhesion
Cell morphology endothelial cell like
Passage characteristics can be passed down2-3generation
digestive juice0.25%
Cultivation conditions gas phase: air,95%；CO2，5%
Cell passage and cryopreservation:
| Cell passage steps | Cell cryopreservation steps |
| If the cell density reaches 80% -90%, subculture can be carried out. 1. Discard the culture supernatant and rinse the cells 1-2 times with PBS that does not contain calcium or magnesium ions. 2. Add 2 ml of digestion solution (0.25% Trypsin 0.53mM EDTA) to a culture bottle and place it in a 37 ℃ incubator for digestion for 1-2 minutes. Then observe the cell digestion under a microscope. If most of the cells become round and fall off, quickly take them back to the workstation, tap the culture bottle a few times, and add a small amount of culture medium to terminate digestion. 3. Add 6-8ml/bottle of culture medium, gently mix and aspirate, centrifuge at 1000RPM for 4 minutes, discard the supernatant, add 1-2mL of culture medium and blow evenly. 4. Divide the cell suspension into new dishes or bottles containing 8ml of culture medium in a ratio of 1:2 to 1:5. | When the cell growth is in good condition, cell cryopreservation can be performed. Taking T25 bottles as an example below; When cells are frozen, discard the culture medium, wash the bottom of the bottle 1-2 times with PBS, and then add 1ml. After the cells become round and fall off, add 2ml of culture medium to terminate digestion, which can be counted using a hemocytometer. Centrifuge at 2000 RPM for 5 minutes to remove the supernatant. Resuspend with serum and add DMSO to a final concentration of 10%. After adding DMSO, quickly mix well and distribute it into cryovials in quantities of 1ml each. Pay attention to labeling the cryovials properly. Our company freezes more than 1X106 cells per freezing tube. 3. Place the cryovial in a program cooling box and store it in a -80 degree freezer for at least 2 hours before transferring it to liquid nitrogen storage. Record the location of the freezer for future retrieval. |
There are generally four methods for culturing primary cells:
① Organizational block cultivation method
Tissue block culture is a commonly used, simple, and highly successful primary culture method. The basic method is to inoculate the cut small tissue clusters into a culture bottle (or dish), and the bottle wall can be pre coated with a thin layer of collagen to facilitate the adhesion of the tissue blocks to the bottle wall, allowing surrounding cells to grow outward along the bottle wall.
② Digestive cultivation method
③ Suspension cell culture method
For cells that grow in suspension, such as leukemia cells, lymphocytes, bone marrow cells, cancer cells and immune cells in pleural and ascites, digestion is not required. They can be isolated by low-speed centrifugation and cultured directly, or cultured by inoculating after lymphocyte stratification.
④ Organ culture
Organ culture refers to the direct cultivation of organs or tissue blocks obtained from donors under specific environmental conditions outside the body without tissue separation. Organ culture can maintain the relative integrity of organ tissues and can be used to focus on observing the connections, arrangements, and interactions between cells, as well as the biological regulatory effects of local environments.
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