Obesity and Fatty Liver Research Platform Animal Experiment Services

Obesity and Fatty Liver Research Platform Animal Experiment Services

OBESITY AND FATTY LIVER RESEARCH PL ATFORM

Construction of Obese Mouse Model

Diet induction can successfully establish a mouse obesity disease model,High fat diet can lead to weight gain in mice with obesity tendency

plus,This model is similar to the clinical pathology of obesity,And it has the advantages of good stability, simple operation, and low cost. In addition,ob/obMouse is a widely used obesity spontaneous mutation mouse model,By leptin( leptin )Genetic point mutations lead toleptinSignal pathway disorders,Therefore, the body cannot produceleptincaused by,Its characteristics are obesity, overeating, high blood sugar, and insulin resistance.

Construction of non-alcoholic fatty liver disease mouse model

Non alcoholic fatty liver induced by high-fat diet( Non-alcoholic Fatty Liver Diseases, NAFLD )Model in

Pathogenesis related to humansNAFLDsimilar,And it often coexists with obesity, insulin resistance, and other metabolic syndromes.HFDDiet and feedingC57BL/6Jmouse7Significant lipid metabolism disorders will appear after a week,The liver has diffuse steatosis accompanied by local inflammatory reactions.

Construction of non-alcoholic steatohepatitis mouse model

Nutritional induced animal model(Food feeding method)isNASHCommonly used animal model establishmentThe method. Among them, methionine/danjian

Lack of feed( methionine-choline deficient diet，MCD )And high-fat feedHFD ( high-fat diet )To induceNASHTwo common types of feed,It can be modeled according to various methods specialSelect appropriate methods for investigation, cycle, animal type, etc.MCDisestablishNASHMethods of animal modeling,Already has40Years of application history,It's researchNASHDisease related inflammation, oxidative stress, mitochondrial damage, and liver fibrosis are preferredModeling methods.

Obesity and Fatty Liver Research Platform Animal Experiment Services