Single component TMB color solution

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

The single component TMB color solution adopts a new single solution TMB color development technology, which catalyzes TMB color development through horseradish peroxidase (HRP), providing low background and high sensitivity color development effects for ELISA and other experiments.

Product Details

Single componentTMBchromogenic solution

Product Description

Single componentTMBThe color developing solution adopts a new single solutionTMBColor development technology, using horseradish peroxidase（HRP）CatalysisTMBColor rendering, forELISAWait for the experiment to provide a low background, high sensitivity color rendering effect.TMB（3,3´,5,5´-Tetramethylbenzidine isHRPCommonly used substrates, inHRPUnder the catalysis of other appropriate peroxidases,TMBGenerate soluble blue products, with absorbance typically ranging from370nmor620-650nmMeasurement within the scope. After the color reaction, it can be achieved by addingTMBColor termination solution（450nm）TheTMBColor termination solution（650nm）To terminate the reaction. use450nmAfter terminating the solution or sulfuric acid, the solution turns yellow and can be dissolved in450nmMeasure absorbance; use650nmAfter terminating the solution, the solution remains blue and can be620-650nmMeasure absorbance.

This colorimetric solution is a ready to use single component solution that does not require the addition of hydrogen or other reagents, solving the problem of traditional multi-component solutionsTMBColor reagents are prone to precipitation and unstable detection results, which simplifies the operation while ensuring the stability and reliability of the detection results. Applicable to37Color development time under temperature conditions or at room temperature15-30minThe testing experiment.

Order Information

Product Name	Item Number	specification
single-componentTMBchromogenic solution	AP37L105	100 mL
single-componentTMBchromogenic solution	AP37L106	500 mL

Transportation and storage

Blue ice transportation.4℃ storage, expiration date48A month.

Product parameters

pHvalue	3.6±0.4
preservative:	0.0002%（w/v）Isothiazolinone
Application:	ELISA

Usage

ELISAColor development steps:

Before use, please prepare the single componentTMBThe color developing solution is equilibrated to the reaction temperature.

450nmRead value:

1. Wash the board, inHRPAfter incubation with enzyme-linked immunosorbent assay, usePBST/TBST washing buffer(Wash the board with surfactants3Secondly;

2. Color rendering, adding100μLSubstrate, according to experimental requirements, in20~37Incubate at ℃ in the dark2-15minOr shorter/For a long time, until the color develops to the expected depth, the color will appear blue.

3. Termination: Join100μLTMBColor termination solution（450nm）(Item number:AP37L125）, turning yellow in color;

4. Reading: Recommended in30 minInternal reading wavelength450The absorbance value at the location;

650nmRead value:

1. Wash the board,HRPAfter incubation with enzyme-linked immunosorbent assay, usePBST/TBST washing buffer(Wash the board with surfactants3Secondly;

3. Termination: Join100μLTMBColor termination solution（650nm）(Item number:AP37L135）The color is blue;

4. Reading: Recommended in30 minInternal reading wavelength650The absorbance value at the location;

dynamics650nmRead value:

1. Wash the board,HRPAfter incubation with enzyme-linked immunosorbent assay, usePBST/TBST washing buffer(Wash the board with surfactants3Secondly;

2. Color rendering, adding100μLSubstrate, in20~37℃ incubation; [Note]: The incubation time is3-30minUntil the color appears to the expected depth.

3. Reading: According to a predetermined frequency (such as every1 min）Read wavelength650nmThe absorbance value at the location.

Precautions

1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

2. Before use, rinse repeatedly with purified water to ensure that a clean container is used to hold the substrate solution. Do not pour the remaining substrate solution back into the original bottle.

3. Suggest using our company's termination solution that does not contain strong acid components for termination, or alternatively0.5M HCLor0.25 M H₂SO₄Terminate the solution.

4. If there is overexposure or precipitation, it indicatesTMBThe substrate reaction is too strong, and the reaction time can be appropriately shortened or the primary antibody can be further diluted/orHRPCombination.