Aladdin TMB color solution B solution (peroxide solution)

Aladdin T117928-25ml TMBChromogenic solutionBLiquid (peroxide solution))

Aladdin TMB color solution B solution (peroxide solution)-Standing stock

Product Item Number:T117928-25ml

Product Name:TMBChromogenic solutionBLiquid (peroxide solution))

Product Specifications25ml

Product Introduction:

English alias:3,3′,5,5′-Tetramethylbenzidine Liquid Substrate System TMB

English name:TMB color reagent B solution (peroxide solution)

Storage temperature:2-8°CStorage, argon filling

Transportation conditions: Ice pack transportation

The specific product parameters are subject to the parameters in the product manual received

3,3 ', 5,5' - Tetramethylbenzidine (TMB) is a peroxidase substrate used forELISAReaction. The substrate produces a soluble light blue end product that can be used in370 or655 nmRead on a spectrophotometer.TMBThe reaction can be used2 M H2SO4 (Generate yellow color)Termination,450 nmRead.TMBIt is divided into soluble and precipitated types. Soluble type: withHRPReaction forms soluble blue brown product, suitable forELISAAnd other liquid reaction detection methodsHRPThe application, andHRPThe reaction forms a precipitated blue brown product, which is a one-step ready to use solution that does not require mixing and is convenient and fast. Precipitation type: suitable forWestern BlotAnd its immunoprecipitation chemistry, one-step ready to use solution, no need for mixing, convenient and fast single componentTMBUnder normal circumstances, peroxidase substrates are light yellow, light blue, or light green. The substrate solution is in650nmTime detectionODValue less than0.04Usage: Mix equally as needed, per well100ulPlease mix and use immediately, do not exceed1Hours, otherwise sensitivity will decrease. Incubation: After adding the above-mentioned solution, incubation can be carried out according to individual experimental needs15－30minute Termination: Adding equal volume1NofHClor2NofH2SO4To terminate the reaction. The reaction solution in the enzyme labeled well changed from blue to yellow. Reading: After terminating the reaction,30Within minutes450nmAttention to reading: Reduce substrate strength: If there is a high reaction background and/Or precipitation, indicatingTMBThe substrate reaction is too strong. To avoid precipitation, readings can be taken immediately after adding the termination solution; Additionally, the primary antibody can be further diluted/orHRPCombination. To reduce non-specific activity in horseradish peroxidaseHRPAmong the several color reaction substrates,TMBDue to its high sensitivity and non carcinogenicity, it has been widely used. This product uses a stable and sensitive formula, and produces a dark blue product during the reaction of horseradish peroxidase conjugates. It is suitable for quantitative and qualitative enzyme-linked immunosorbent analysis, but not for membrane staining or immunohistochemical staining.

Aladdin TMB color solution B solution (peroxide solution)-Standing stock

Product ordering information:

T117928-25ml TMB color solution B solution (peroxide solution) 25ml

T117928-100ml TMB color solution B solution (peroxide solution) 100ml

T117928-500ml TMB color solution B (peroxide solution) 500ml