Extraction of blood RNA and DNA using a small amount extraction kit

Blood RNA DNA Small Volume Extraction Kit Extraction Series

The kit consists of blood RNA&DNA protective solution (Buffer BR1, Cat # RP601), which facilitates the collection, transportation, and storage of samples in batches.

After mixing the blood sample with Buffer BR1 in a ratio of 1:2.5 and leaving it at room temperature for 2 hours, the DNA and RNA in the sample solidified into powder like aggregates that were easily centrifuged and precipitated.

DNA adsorption column RD selectively adsorbs DNA and retains RNA in the filtrate. After adding ethanol to the filtrate to adjust the binding conditions, RNA adsorption column-A effectively adsorbs large molecules of RNA (including mRNA and rRNA, excluding tRNA and small RNAs degraded to 5S).

After washing the DNA adsorption column RD and RNA adsorption column A separately, use deionized water or low salt solution to wash the DNA and RNA separately, which can be directly used for subsequent experiments.

This kit is suitable for extracting DNA and RNA from ≤ 1ml fresh human or mammalian whole blood and ≤ 3ml frozen human or mammalian whole blood, respectively. It is suitable for processing EDTA-K2 anticoagulant blood and not suitable for processing heparin or citric acid anticoagulant blood.

RNA production and application: 3-8 μ g RNA/ml fresh blood, 1-2 μ g RNA/ml frozen blood; A small elution volume of 15 μ l. Most of the tRNA and small RNAs degraded into 5S have been removed and can be directly used for 5 ` RACE and 3 ` RACE.

DNA production and application: 5-10 μ g DNA/ml blood; A small elution volume of 50 μ l. The purity is lower than that of the blood genomic DNA extraction series kit, which can be used for enzyme digestion and routine PCR.

This kit is also suitable for extracting viral RNA and DNA from animal feces and intestinal contents of mollusks.
The sample was dispersed in physiological saline, and the supernatant from centrifugation contained viral RNA and DNA. After mixing the centrifuged supernatant with Buffer BR1 in a ratio of 1:2.5 and leaving it at room temperature for 2 hours, the DNA and RNA in the sample were solidified into powdery aggregates that were easily precipitated by centrifugation. This process can remove the majority of PCR inhibitory components. The subsequent operation is the same as processing blood samples.

Extract RNA from 0.4ml fresh whole blood

Elution volume 25 μ l, electrophoresis volume 2 μ l, 1 × TAE, 2% agarose, 6.7 V/cm for 15 minutes

The RNA obtained from fresh blood has no obvious genomic DNA and 5S RNA.

In non denatured agarose gel, the mobility of 28S RNA, 18S RNA and 5S RNA was similar to that of 2000 bp DNA, 750 bp and 100 bp DNA, respectively.

Blood RNA DNA Small Volume Extraction Kit Extraction SeriesChuangling Biotechnology provides spot supply!