Yuan Cai pre dyed protein marker (10-250KD)

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

Yuancai pre stained protein marker (10-250KD) contains 10 highly purified and pre stained recombinant proteins (10, 15, 25, 35, 40, 55, 70, 100, 130, 250KD). Among them, the 70KD band is orange red, 10KD is green, and the remaining bands are blue, which are suitable as protein molecular weight standards for SDS-PAGE and Western Blot.

Product Details

Yuan Cai pre dyed proteinMarker（10-250KD）

Product Description

YuancaiPre stained proteinMarker（10-250KD）It includes10Highly purified and pre stained recombinant protein（10、15、25、35、40、55、70、100、130The250KD）Among them,70KDThe strip is orange red in color,10KDIt is green, and the other stripes are blue,Suitable asSDS-PAGEandWestern BlotThe protein molecular weight standard.

This product does not containHis，Can be used directly without boiling, diluting, or adding reducing agents. According to the size of the sample hole, sample each time3-5μLVery clear protein bands can be observed during electrophoresis, after electrophoresis, and after membrane transfer.

Order Information

Product Name	Item Number	specification
Yuan Cai pre dyed proteinMarker（10-250KD）	AP13L719	250ML
Yuan Cai pre dyed proteinMarker（10-250KD）	AP13L720	2*250μL

Transportation and storage

Blue ice transportation.4℃Short term storage, validity period2Months;-20℃Long term storage, expiration date36A month.

Usage

1. Thawing: Remove the protein at room temperatureMarkerThaw, or use warm hands to quickly thaw it.

2. Mixing: Use a vortex oscillator to gently mix, ensuring that the solution is homogeneous.

3. Sample above:

(1) For small-sized adhesive(mini-gel)Suggestion to sample5 μLUsed for clear strip display.

(2) For large adhesives(large-gel)The sample size can be increased as appropriate.

Precautions

1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

2. PAGEThe pore size of gel will change with the change of gel concentration. Low concentration gel has larger pore size, and small molecular weight protein will migrate faster than the dye front edge. In order to better separate small molecular weight proteins, it is recommended to use higher concentration of gel; For the separation of high molecular weight proteins, the lower concentration of gel is more suitable.

3. When transferring high molecular weight proteins, it is recommended to extend the transfer time or increase the transfer voltage. If the effect is not good, it is recommended to adjust the transfer solution formula, reduce the amount of methanol used, and add a small amountSDSThe final concentration shall not exceed0.1%）.