Yuan Cai pre dyed protein marker (10-180KD)

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

Yuancai pre stained protein marker (10-180KD) contains 10 highly purified and pre stained recombinant proteins (10, 15, 25, 35, 40, 55, 70, 100, 130, 180KD). Among them, the 70 and 25KD bands are orange, 10KD is green, and the remaining bands are blue, which are suitable as protein molecular weight standards for SDS-PAGE and Western Blot.

Product Details

Yuan Cai pre dyed proteinMarker（10-180KD）

Product Description

YuancaiPre stained proteinMarker（10-180KD）It includes10Highly purified and pre stained recombinant protein（10、15、25、35、40、55、70、100、130The180KD）. among which,70The25KDThe strip is orange,10KDIt is green, and the other stripes are blueSuitable asSDS-PAGEandWestern BlotThe protein molecular weight standard.

This product does not containHisIt can be used directly without boiling, diluting or adding reducing agents. According to the size of the sample hole, sample each time3-5MLVery clear protein bands can be observed during electrophoresis, after electrophoresis, and after membrane transfer.

Order Information

Product Name	Item Number	specification
Yuan Cai pre dyed proteinMarker（10-180KD）	AP13L616	250ML
Yuan Cai pre dyed proteinMarker（10-180KD）	AP13L617	2*250μL

Transportation and storage

Blue ice transportation.4Short term storage at ℃, expiration date2Months;-20Long term storage at ℃, expiration date36A month.

Usage

1. Thawing: Remove the protein at room temperatureMarkerThaw, or use warm hands to quickly thaw it.

2. Mixing: Use a vortex oscillator to gently mix, ensuring that the solution is homogeneous.

3. Sample above:

(1) For small-sized adhesive(mini-gel)Suggestion to sample5 µLUsed for clear strip display.

(2) For large adhesives(large-gel)The sample size can be increased as appropriate.

Precautions

1. This product is only for scientific experimental research and should not be used in clinical diagnosis, treatment, or other fields.

2. PAGE(Polyacrylamide gel electrophoresis) The gel pore size will change with the change of gel concentration. Low concentration gel has larger pore size, and small molecular weight protein will migrate faster than the dye front edge. In order to better separate small molecular weight proteins, it is recommended to use higher concentration of gel; For the separation of high molecular weight proteins, the lower concentration of gel is more suitable.

3. inWestern blotIn experiments, for high molecular weight proteins, longer membrane transfer times or higher voltages are usually required.