Pure imported CFX Opus 96 fluorescence quantification 12011319

CFX opus 96Real time fluorescence quantificationPCRceremony#12011319

Overview

96 Hole5 Color PlusFRETReal time network connectivityPCRDetection system, includingCFX opus 96Basic unit, cable

CFX opus 96Real time fluorescence quantificationPCRceremony#12011319

description

CFX opusIt belongs to us *CFXNew real-time updates in the seriesPCRSystem.

CFX opus 96Real time fluorescence quantificationPCRThe system can analyze96 A hole and provides many new features:

More uniform thermal performance

Extended connectivityWi-FiEthernet andUSB

Cloud Connection: in ourBR.ioCloud platform or desktopCFX MaestroSoftware operationCFX opus

Network storage drive access for excellent data management

The shuttle optical system produces consistent optical measurements on your sample board

CFX opus 96Real time fluorescence quantificationPCRceremony#12011319

technical indicators

PCRceremony

Heating and cooling rate,°C/sec 5

The average rate of temperature rise and fall,°C/sec 3.3

Heating and cooling methods Peltier

lid, °C 30–110

temperature

Range,°C 4–100

Accuracy:°C 90°CTime programming objective ±0.2

Uniformity:°Creach the goal10 Within seconds ±0.3 Hole to hole

gradient

Scope of work,°C 30–100

Programmable range,°C 1–24

optical inspection

motivation 6 A filtered oneLED

detection 6A filtering photodiode

Excitation wavelength range,nm 450–684

Emission wavelength range,nm 510–730

sensitivity Detecting the human genome DNAin1 Copy of target sequence

dynamic range 10An order of magnitude

scan time

All channels, in seconds 12

only FAM/SYBR® Green, seconds3

software

operating system Windows 10

Multiple analysis Each well can reach 5 a goal

system

Sample load, pore size 96

Sample size,µl 1–50(Recommended)10–50）

communication interface USB 2.0

Electrical certification The International Electrotechnical Commission,CE

Size (width) xdeepxHeight, centimeters/Inches33x56x36 / 13x22x14

Weight, kg/Pound22/48

1、 Basic Information

Model:CFX opus 96

Manufacturer:Bio-rad(Bole)

2、 Basic functions

Accurate temperature control can be achieved to amplify nucleic acid fragments and accurately quantify fluorescence signals.

3、 Basic specifications and propertiesAbility and parameter indicators:

1. Detection channels: ≥ 6

2. Reagent consumables open: can use 0.2ml single tube, eight tube, 96 well plate, supporting various reagents

3. Suitable for various fluorescence methods, such as Taqman, Molecular Beacon, FRET probe, SYBR Green I, etc

4. Touch screen user interface: The screen can be adjusted 12-55 degrees according to user usage, and experimental programs can be created and modified.

5. Sample capacity: 96 × 0.2ml

6. Reaction system: 1-50 µ l

7. Light source: ≥ 6, each channel has an independent light source, for example: four channels with four independent light sources are excited to avoid mutual interference of light paths.

8. Excitation wavelength: 450nm-684nm

9. Detector: ≥ 6, photomultiplier tube or photodiode

10. Detection wavelength: 510nm-730nm

11. Maximum heating and cooling rate: ≥ 5 ℃/s

12. Temperature control accuracy: ≤± 0.2 ℃

13. Temperature uniformity: ≤± 0.3 ℃

14. Heating module temperature control range: 4-100 ℃

15. Hot lid temperature control range: 30-110 ℃

16. Temperature gradient function: It can operate at more than 6 different annealing temperatures simultaneously, with a temperature difference control range of 1-24 ℃, which helps optimize experimental conditions.

17. Control mode: External computer control, independent operation controlled by the host touch screen.

18. Cloud data service: access the cloud based experimental settings and management data through the Internet at any time, send the completed experimental results through e-mail, and save the experimental procedures and results in the network folder.

19. Data transmission method: Data can be transmitted through WiFi, network, or USB.

20. Data analysis modes: absolute quantification, relative quantification, multi reference multi gene analysis with amplification efficiency correction, no data file merging analysis, and allele analysis.

21. Statistical analysis functions: T-test analysis, analysis of variance, bar chart, cluster chart, scatter plot, heatmap, volcano plot, etc

22. Data export: It can save, copy, and print all data and forms in the result analysis interface, export specific data in multiple formats, and directly copy and paste them into commonly used office software such as Word, Excel, or PowerPoint.

23. Image output: 600dpi high-definition images for literature publication can be directly exported, supporting common BMP, JPG, and PNG file formats for output.

24. Calibration function: No need for regular fluorescent dye calibration or lifetime free fluorescent dye calibration provided by the manufacturer.