Flow cytometry detection Flow cytometry antibody BD FACS Diva CS& T Research Beads Microspheres

Becton Dickinson Bioscience, BD Company, USA Flow Control Product Introduction:
Flow cytometry detection Flow cytometry antibody BD FACS Diva CS&T Research Beads microspheresCommon item numbers: BD340486, BD335775, BD345249, BD650621, BD653144, BD342003; BD flow cytometer quality control microspheres make flow cytometry detection results more reliable.

BD (Bidi) is one of the medical technology companies that develops, produces, and sells medical equipment, medical systems, and reagents, committed to improving human health. BD (Bidi) focuses on improving drug delivery, enhancing the quality and speed of infectious disease and cancer diagnosis, and promoting the research and production of new drugs and vaccines. BD (Bidi) company has * research and development capabilities. The company was founded in New York in 1897 and is headquartered in Franklin Lake, New Jersey, USA. Its business covers *. The company's business can be divided into three categories: BD Medical, BD Diagnostics, and BD Bioscience. Production and sales include medical consumables, laboratory instruments, antibodies, reagents, diagnostics, and other products. The company's service targets include medical institutions, life science research institutes, clinical laboratories, industrial units, and the general public.
The working principle of flow cytometry (FCM) is to perform multi parameter and rapid quantitative analysis of individual cells or other biological particles at the cellular molecular level using monoclonal antibodies. It can analyze tens of thousands of cells at high speed and simultaneously measure multiple parameters from one cell, with the advantages of fast speed, high accuracy, and good precision. The main components of the flow cytometer are the light source, fluid flow pathway, signal detection and transmission, and data analysis system. At present, the use of flow cytometry for the detection of peripheral blood white blood cells, bone marrow cells, and tumor cells is an important component of clinical testing.

Flow cytometry (FCM) is increasingly being used in clinical testing. Due to the nature of clinical testing itself, it requires managers and operators of clinical testing to ensure the quality of routine operations and result analysis. At the same time, one must also know how to determine whether the score is under control or out of control. As a clinical laboratory work, the quality standard of its work is to ensure that the test results are in line with the actual situation of whether the patient has lesions or not. In clinical testing, quality control is divided into pre analysis, in analysis, and post analysis. The main content of quality control before analysis includes specimen collection, preservation, and transportation; Quality control in analysis refers to the control of the experimental operation process; The quality control after analysis mainly involves processing data results, evaluating the credibility of inspection results, and promptly sending reports to clinical practice and listening to feedback.
Flow cytometry (FCM), as an excellent detection technique, is no exception for quality control. At present, the application of flow cytometry (FCM) in clinical testing mainly focuses on several aspects: 1. Immunophenotype analysis, including peripheral blood lymphocyte phenotype analysis, leukemia/lymphoma immunophenotyping, platelet membrane glycoprotein analysis, HLA-B27 detection, paroxysmal hemoglobinuria (PNH) detection, etc. 2. Cell DNA and RNA detection and cell cycle analysis, including DNA ploidy detection, cell cycle analysis, reticulocyte detection, reticulocyte platelet detection, etc. 3. Quantitative analysis, including absolute quantification of peripheral blood CD4 in AIDS classification, absolute quantification of CD3 in OKT3 treatment monitoring, quantification of CD34 in stem cell transplantation, and so on. The quality control of flow cytometry (FCM) experiments naturally includes the quality control of the entire experimental process, such as instrument and equipment, sample processing, reagents, operating procedures, and data analysis.

Flow cytometry detection Flow cytometry antibody BD FACS Diva CS&T Research Beads microspheresQuality Control:
The calibration of a flow cytometer includes the stability of the flow path, the stability of the optical path, multi-color labeling fluorescence color compensation, and the linearity and stability of the photomultiplier tube conversion. The calibration of the instrument mainly uses standard microspheres for monitoring. Polystyrene can be made into microspheres of various sizes, and can also be fluorescently labeled or have binding sites for quantitative immunoglobulin. This polystyrene microsphere with fixed fluorescence intensity, size, and light scattering properties has become a commonly used standard in flow quality control.
Platelet testing, etc. 3. Quantitative analysis, including absolute quantification of peripheral blood CD4 in AIDS classification, absolute quantification of CD3 in OKT3 treatment monitoring, quantification of CD34 in stem cell transplantation, and so on. The quality control of flow cytometry (FCM) experiments naturally includes the quality control of the entire experimental process, such as instrument and equipment, sample processing, reagents, operating procedures, and data analysis.

BD company has all the standard microsphere quality control products and daily maintenance products required for instrument calibration, as shown in the table below: