Ceramic BCA protein quantification kit - readily available in stock

Pottery technique C0050 BCAProtein quantification kit

Ceramic BCA protein quantification kit - readily available in stock

Product Item Number:C0050

Product Name:BCAProtein quantification kit

Product Specifications500T/2000T/4000T

Product Introduction:

BCA（Bicinchoninic acid）It is the most widely used protein concentration determination method, commonly used for quantitative detection after protein extraction. It has the advantages of simple operation, good stability, high sensitivity, and high compatibility.

BCAThe detection principle of the protein concentration determination kit involves a two-step chemical reaction. The first step is based on the biuret reaction, in which the protein forms a complex with divalent copper ions in an alkaline environment and reduces them to monovalent copper ions, forming a blue complex. The second step isBCACombined with monovalent copper ions, a stable blue purple complex is formed, which is562 nmThere is a high absorbance value at this point, which is proportional to the protein concentration.

The specific product parameters are subject to the parameters in the product manual received

Product Composition

C0050-1 BCAreagentA 100mL

C0050-2 BCAreagentB 3mL

C0050-3 BSAProtein standard 30mg

C0050-4 Protein standard preparation solution 1.5mL

High sensitivity: the minimum amount of detected protein reaches0.5 MgThe detection concentration has reached the lower limit25 Mg/mLThe volume of the sample to be tested is1-20ML.

Wide linear range: in25-2000Mg/mLThere is a good linear relationship within the concentration range.

Easy to operate: Under normal circumstances, it can be used45Complete the measurement within minutes, better than the classicLowryThe method is four times faster.

Good compatibility:BCAThe method for determining protein concentration is not affected by the majority of chemical substances in the sample and can be compatible with up to5%ofSDS，5%ofTriton X-100，5%ofTween 20、60、80However, this reagent kit is affected by chelating agents and slightly higher concentrations of reducing agents, so it is necessary to ensure thatEDTAbelow10mM, noneEGTA，DTTbelow1mMand β-Mercaptoethanolbelow0.01%.

In the reagent kitATheBLiquid and protein standard preparation solution should be stored at room temperature.

Protein standard（BSA）The powder is stored at room temperature and can be placed in a solution after preparation-20Store at ℃ for one year.

This method is suitable for use96The orifice plate is detected using an enzyme-linked immunosorbent assay (ELISA) reader. If there is no enzyme-linked immunosorbent assay (ELISA) reader, a regular spectrophotometer can also be used for measurement, but during the measurement, it is necessary to increase the detection volume appropriately according to the minimum detection volume of the colorimetric dishBCAThe dosage of working fluid, standard samples, and samples.

If reagents are found during low-temperature or long-term storageAOr reagentsBThere is sediment, please37ºCIncubate and stir until fully dissolved. If bacterial contamination is found, it should be discarded to avoid affecting the experimental results.

It is recommended to make corresponding standard curves for each measurement, as the color reaction is related to changes in temperature and time, and accurate protein quantification should be done with standard curves every time.

This product is only for scientific research by professionals and should not be used for clinical diagnosis or treatment, food or medicine, or stored in ordinary residential areas.

For your safety and health, please wear lab coats and disposable gloves when operating.

Ceramic BCA protein quantification kit - readily available in stock

Product ordering information:

C0050 BCAProtein quantification kit 500T/2000T/4000T

For other item numbers, please consult our store customer service