Ceramic Cell Counting Kit-8 CCK-8- readily available in stock

Pottery technique C0005 Cell Counting Kit-8 （CCK-8）

Ceramic Cell Counting Kit-8 CCK-8- readily available in stock

Product Item Number:C0005

Product Name: Cell Counting Kit-8 （CCK-8）

Product Specifications1ml/5ml/10ml/30ml/100ml

Product Introduction:

The Cell Counting Kit-8 (CCK-8) kit uses water-soluble tetrazolium saltsWST-8 (Chemical name:2-(2-methoxy-4-Nitrophenyl group)-3-(4-Nitrophenyl group)-5-(2,4-Disulfobenzene)-2H-Tetrazole monosodium salt)The principle of achieving rapid detection isWST-8In the presence of electron coupling reagents, it can be reduced by dehydrogenases within mitochondria to produce orange yellow methylene dye（formazan）. By colorimetric analysis, the number of live cells can be dynamically quantified to detect cell proliferation or drug toxicity.CCK-8The solution can be directly added to cells without pre mixing with other components, making it easy to operate and accurate to detect.

The specific product parameters are subject to the parameters in the product manual received

The detection sensitivity of CCK-8 is higher than other methods, such asMTT，XTTorWST-1；

The operation is simpler and does not require organic solvents;

Non toxicity to cells;

Stable reagents, ready to use.

Cell proliferation assay

Cell viability testing

Cytokine experiment

Cytotoxicity experiment

Operating instructions

1. Inoculate cell suspension100MLYu96Orifice plate, in a cell culture incubator（37°C, 5% CO2）Incubation.

2. Remove the cells to be tested and add them directly to the cell culture medium1/10volumetricCell Counting Kit-8 (CCK-8)Mix thoroughly to ensure color uniformity in the pores, but no bubbles should be generated. right96Orifice plate, each100µlAddition of culture medium10µltest kit.

3. Continue to cultivate in the cell culture box1-4Hour.

Before reading the enzyme-linked immunosorbent assay (ELISA) reader, place it on the well plate and shake it on the shaker for about1minEnsure that the color of the orifice plate is uniform.

5. Read with an enzyme-linked immunosorbent assay reader450nmLight absorption value, calculate cell activity.

cell viability=[(experimental group-blank group) / (control group-blank group)]×100%

Cell inhibition rate=[(control group-experimental group) / (control group-blank group)]×100%

Experimental group: including cells, culture mediumCCK-8And drugs;

Control group: containing cells, culture mediumCCK-8, without medication;

Blank group: containing culture mediumCCK-8It does not contain cells or drugs.

6. Optional steps: In100MlAdd to cells10 Ml 3 % SDSTo terminate the reaction, it can be done3Read data within days.

Storage:4°CIt can be stored for one year in the dark,-20°CIt can be stored for two years in the dark.

Precautions

Please mix thoroughly before use to avoid repeated freezing and thawing

Pay attention to the edge effect of the 96 hole plate. It is recommended to abandon the surrounding circle of plate holes and add the same amountPBS.

This product can only be used for research and is strictly prohibited from being used on the human body.

Ceramic Cell Counting Kit-8 CCK-8- readily available in stock

Product ordering information:

C0005 Cell Counting Kit-8 (CCK-8) 1ml/5ml/10ml/30ml/100ml

For other item numbers, please consult our store customer service