Extracellular vesicle isolation kit (urine) (50 mL)

NegotiableUpdate on 05/06

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Nature of the Manufacturer: Producers

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Overview

Suzhou Haibosisi Biotechnology Co., Ltd. ("Haibosisi") is located in Fenhu Science and Technology Innovation Park, Wujiang District, Suzhou City. It focuses on the research and development, production, sales, and technical services of biological product quality control and testing products, as well as core raw materials in the life science industry chain. Our core products cover multiple categories, including process impurity residue, host cell nucleic acid and protein residue detection, complex sample nucleic acid extraction, PCR, fluorescence quantitative PCR, fragment analysis, cell culture additives, cytokines, and customized gene detection technology services. Haibosisi uses molecular biotechnology as a platform, and we strictly control the production process and fully implement quality monitoring

Product Details

Solution for extracellular vesicle RNA isolation and detection

Extracellular vesicle RNA, especially small RNAs such as miRNA, are potential biomarkers and functional regulatory molecules. Its research faces three major challenges: extremely low content, easy degradation, and background interference. We provide targeted full process tools aimed at efficiently obtaining real and reliable data.

1、 RNA isolation: efficient capture, high-purity acquisition

The key to success lies in fully cleaving the extracellular vesicle membrane and efficiently recovering trace amounts of nucleic acid.

Efficient Cracking and Extraction Kit

Core advantage: The lysis solution needle optimizes the extracellular vesicle lipid bilayer membrane, allowing for the full release of its contents; Containing a unique carrier RNA, it can significantly reduce the wall adsorption loss of trace RNA during the extraction process and improve the recovery rate.

Remove interference: It can effectively separate and remove free proteins and genomic DNA background in the sample, avoiding false positives in downstream detection.

Scope of application: Compatible with multiple separation methods (ultracentrifugation, precipitation, size exclusion, etc.) to obtain extracellular vesicle precipitates or solutions.

MiRNA/small RNA enrichment extraction column

Specific binding: The silica gel membrane is specially treated to have high affinity for small RNA fragments<200nt, enabling selective enrichment of small RNAs (miRNA, piRNA, tRNA fragments, etc.).

Rapid purification: Short operation time, reduced degradation risk, elution volume can be as low as 10 μ L, making it easy to obtain high concentration RNA.

2、 RNA Quality Control and Quantification: Confirming Quantity and Integrity

Before entering the functional testing, a basic evaluation of the extract is required.

High sensitivity fluorescent quantitative reagent

Suitable for trace samples, only 1 μ L is needed to accurately quantify the total RNA concentration, with much higher sensitivity than UV spectrophotometry.

Small RNA integrity electrophoresis analysis reagent

Provide specialized electrophoretic markers and dyes to clearly display the 18-26nt miRNA main band and snoRNA marker bands, allowing for intuitive assessment of RNA integrity and the presence of large RNA contamination.

3、 RNA detection and analysis: from validation to discovery

Provide multiple sensitive and specific detection tools based on research objectives.

Reverse transcription and qPCR detection (for known targets)

Universal Stem Ring Reverse Transcription Kit: Designed with stem ring primers, it significantly improves the reverse transcription specificity and efficiency for short chain miRNAs, making it the gold standard method for micro detection.

Pre plate/Primer Pool: Provides miRNA pre well plates or multiple primer pools related to common diseases or signaling pathways, facilitating rapid expression profiling screening.

Universal high-sensitivity qPCR premix: It has strong adaptability to complex cDNA templates, high amplification efficiency, good repeatability, and can stably detect extremely low abundance targets with Ct values>35.

Small RNA sequencing library construction (for unknown discoveries and panoramic analysis)

3 'Terminal Connector Optimization Library Kit: Targeting the characteristics of short and modified extracellular vesicle RNA fragments, optimizing connection efficiency, reducing preference, and accurately restoring RNA population composition.

Removing rRNA and tRNA backgrounds: The built-in probe can efficiently remove high abundance structural RNA, increasing the proportion of effective data.

New type of extraction free direct detection technology

Lysis detection integrated reagent: For specific projects (such as rapid screening of plasma extracellular vesicle miRNA), extracellular vesicle lysis can be integrated with qPCR reaction system to achieve "direct detection after separation", greatly shortening the process and avoiding extraction losses.

Summary: Full process support

We understand that every aspect of extracellular vesicle RNA research is crucial. From efficient separation and extraction, to precise quantitative validation, and to deep sequencing discovery, we provide rigorously tested and compatible reagent combinations to ensure that every step from vesicles to data is clear, controllable, and reproducible.

Focusing on micro nucleic acid technology to help you capture key information in extracellular vesicles.