Mouse lymphocyte isolation solution Dayou/Dake is a 4 * 100ml scientific research product

Dayou is an independent brand of Dake, providing a complete set of ELISPOT/ELISA experimental products, serum-free ELISPOT culture medium and DC culture medium; Pre packaged with ELISPOT assay kit and reading board service for humans and mice; Pre packaged ELISA kits for humans, mice, and rats; Human separation liquid/tube and rodent and pig separation liquid. Medical grade separation solution (with medical device license) and separation tube, with low endotoxin content (less than 0.06EU/ml) and good separation effect.
ELISA, ELISPOT training, and sample testing services. Launch the Super series of immune cell culture products for cultivating immune cells such as DCs and CIKs.

Mouse lymphocyte isolation solution Dayou/Dake is a 4 * 100ml scientific research product

Da You ® Mouse lymphocyte isolation solution

All imported materials, *; High purity, good condition, and high yield of isolated cells (up to 5x107-1x108 cells per spleen); Easy to operate, the spleen is directly ground in the separation solution, which can efficiently remove dead cells

【 Scope of application 】 Isolation of lymphocytes from mouse (or rat) spleen; Or isolate PBMCs from rat (or rabbit) blood, but with different methods

Density: 1.0810 ± 0.0005g/mL (20 º C)

Osmotic pressure: 280 ± 15mOsm

【 Main Ingredients 】 Iodixanol, no chemical toxicity, no biological toxicity

0.5EU/mL

【 Storage conditions 】 Store at 4 º C in the dark to maintain sterility

【 Shelf life 】 12 months

【 Principle Introduction 】 Mouse spleens are ground in lymphocyte separation medium, and the cell fragments and dead cells generated during the operation are infiltrated into high-density (1.0810) cell separation medium through internal diffusion. After centrifugal separation, dead cells and cell debris with different densities deposit at the bottom of the tube, and lymphocytes aggregate to form a clear white membrane layer.