Recombinant human bone morphogenetic protein-2 (BMP-2)

Product attributes:

Background:

Bone morphogenetic protein-2（BMP-2）It is an extracellular multifunctional signaling cytokine, and alsoTGF-Members of the beta family.BMP-2can be paired withTGF-β receptor binding, activationSMADThe signal transduction of proteins. In addition, it also participatesWntandNotchWaiting for the signal path.BMP-2Plays an important role in the growth of hard bone and cartilage, and participates in the differentiation of osteoblasts.

Tag:

1. Mix the cell culture medium with EdU solution in a ratio of 500:1 to prepare 2 × EdU labeled culture medium.

2. Preheat the 2 × EdU labeled culture medium in advance, and then mix 150 μ L of 2 × EdU labeled culture medium with an equal volume of cell culture medium to obtain a 1 × EdU solution.

[Note]: ① It is not recommended to replace all the culture media, as this may affect the speed of cell proliferation. ② It is recommended to use the prepared culture medium immediately, and the dosage should not exceed the cells.

3. Incubate the cells for 2 hours and discard the culture medium.

【 Note 】: ① The cultivation time depends on the proliferation rate and growth status of the cells. ② Most tumor cells and adherent cell lines can be incubated for 2 hours.

4. Wash the cells twice with 1xPBS for 5 minutes each time to remove EdU residues that have not been incorporated into DNA.

[Note]: For cells that are not firmly attached to the wall, the cleaning intensity can be reduced.

Experimental procedure:

1. Inoculate cells at a density of 104-105 cells/well into a 96 well plate and add 100 μ L of culture medium, with or without the test compound. Cultivate cells in a CO2 incubator at 37 ℃ for 24 hours.

2. Add 10 μ L of different concentrations of the test substance to the plate.

3. Cultivate the culture plate in the incubator for an appropriate period of time (such as 6, 12, 24, or 48 hours).

4. Add 10 μ L of CCK-8 solution to each well of the plate. Be careful not to introduce bubbles into the hole.

5. Incubate the plate in the incubator for 1-4 hours.

6. Gently mix on the fixed orbit oscillator before reading the board.

7. Measure the absorbance at 450nm using an enzyme-linked immunosorbent assay (ELISA) reader.

The products currently being sold by the company:

FSCN1 (Fascin 1 protein; fascin homolog 1, actin-bundling protein 0.5mgFSCN1 (Fascin 1 protein; fascin homolog 1, actin-bundling protein)Fiber bundle protein homolog1/Actin binding protein/Circular nematode purpura antigen

IL36G Protein HumanRecombinant humanIL36G / IL1F9protein(aa 18-169, Hislabel)

CD7Recombinant ratsCD7protein(Fclabel) Protein

P4HB Protein MouseRecombinant miceP4HB / ERBA2Lprotein

SECTM1Recombinant humanSECTM1 / K12proteinProtein

Guinea pig interleukin18(IL-18)Test kit, English name:IL-18 ELISA Kit

Human ubiquitin ligase (E3/UBPL) ELISA KitHuman ubiquitin ligase(E3/UBPL)Test kit

rabbitIerleukin8,IL-8ELISAKitRabbit interleukin8(IL-8/CXCL8)Test kit96T/48TImport packaging

Cliakibeta-cgelisakitRat chorionic β

Bacterial alcohol dehydrogenase total activity colorimetric quantitative detection kit20time

RabbitFibrinogen,FbgELISAKitRabbit fibrinogen(Fbg)Specification of detection kit:96T/48T

Storage conditions:2-8℃ ⍬⍬

Recombinant human bone morphogenetic protein-2（BMP-2)ACVR2ARecombinant miceACVR2A / ActrIIaprotein(His & Fclabel) Protein

Beta-Amyloid(1-28 0.5mgBeta-Amyloid(1-28)β - amyloid peptide1-28(polypeptide protein)

NRG1Recombinant humanNRG1-alphaprotein(EGF Domain, Fclabel) Protein

ITGAX & ITGB2 Protein HumanRecombinant humanITGAX & ITGB2 Heterodimerprotein

FZD4 Protein RatRecombinant ratsFrizzled-4 / FZD4protein(Fclabel)

Human heterogeneous ribonucleoprotein complex/resistRA33antibody(HNP / RA 33) ELISAtest kit

Human ansfer factor (TF) ELISA KitHuman transfer factor(TF)Test kit

Humahymidylatesyhetase,TSELISAKitHuman TS(TS)Test kit96T/48TImport packaging

HumanIerleukin17,IL-17Detection kit for human interleukin17(IL-17)Specification of detection kit:96T/48T

Organic soluble total protein preparation kit20time

Humaniestinalfattyacidbindingprotein，iFABPELISAKitHuman intestinal fatty acid binding protein(iFABP)Specification of detection kit:96T/48T

Klenow FragmentSpecifications300 units

Klenow FragmentSpecifications1500 units

Klenow Fragment, LCSpecifications300 units

T4 DNA PolymeraseSpecifications100 units

T4 DNA PolymeraseSpecifications500 units

T7 DNA PolymeraseSpecifications300 units

phi29 DNA PolymeraseSpecifications250 units

phi29 DNA PolymeraseSpecifications1000 units

phi29 DNA PolymeraseSpecifications5000 units

T3 RNA PolymeraseSpecifications2000 units

T3 RNA PolymeraseSpecifications10000 units

T3 RNA Polymerase, HCSpecifications10000 units

T7 RNA PolymeraseSpecifications5000 units

T7 RNA PolymeraseSpecifications5x5000 units

T7 RNA Polymerase, HCSpecifications25000 units

SP6 RNA PolymeraseSpecifications2000 units

SP6 RNA Polymerase, HCSpecifications5000 units

Terminal Deoxynucleotidyl TransferaseSpecifications500 units

Terminal Deoxynucleotidyl TransferaseSpecifications2500 units

M-MuLV Reverse TranscriptaseSpecifications1000 units

M-MuLV Reverse TranscriptaseSpecifications5000 units

Taq DNA Polymerase (recombinant)Specifications500 units

Taq DNA Polymerase, LC (recombinant)Specifications500 units

Taq DNA Polymerase (recombinant)Specifications5x500 units

Taq DNA Polymerase (recombinant)Specifications10x500 units

Klenow Fragment, exo–Specifications300 units