New Green Story of Millennium Port City: Implementation and Operation of Chlorophyll Fluorescent Plant Phenotypes at Ningbo University

As the starting point of the Maritime Silk Road, Ningbo's connection with plants has lasted for thousands of years, from sericulture to tea. Recently, the Yiketai Plant Chlorophyll Fluorescence Imaging System has officially settled at Ningbo University. At the same time, it has also conducted follow-up visits and experimental guidance services for some old customers, helping them explore basic research fields from edible crops to deep-sea algaeUsing "chlorophyll fluorescence" as a brush stroke, continue writing a brand new green chapter.

This series of chlorophyll fluorescence imaging systems integratesPAMChlorophyll Fluorescence Technology and High ResolutionCCDImaging has endowed the study of photosynthesis with two-dimensional dynamic observation capabilities. It supports photochemical quenching, non photochemical quenching, etc50Residual fluorescence parameter imaging is suitable for whole plants, including plant tissues with photosynthetic capacity such as leaves and fruits, as well as lower plants and algae samples such as mosses and lichens. Widely used in scientific research fields such as plant optical mechanism research, modern molecular breeding, crop stress and resistance detection, plant disease and case detection, and plant environmental response.

Research application case:

Chili light mottled virus（PMMoV）Infection with Nicotiana benthamiana can cause changes in its photosynthesis, such as changes in the photosystemII（PSII）Inhibition, downregulation of photosynthetic electron transport chain and Calvin cycle related chloroplast proteinsATP/NADPHImbalance of assimilation potential, etc. This study utilizesFluorCamChlorophyll fluorescence imaging was used to analyze photosynthesis and secondary metabolism activation under biological stress, achieving stress detection before symptom onset (Φ)PSIIThe decrease in value provides key information on the migration of pathogens in host plants.

During low tide, when exposed to dehydration pressure in the air, the photosynthetic activity of Porphyra yezoensis rapidly decreases and is lower than that of high salinity and high mannitol concentration treatments.thisResearch has found that high salinity only occurs at maximum water loss（40% WL）It significantly reduces photosynthesis, but maintains a relatively stable statePSIIMaximum quantum yield（Fv/Fm）. High concentration of mannitol treatment leads to20% WLsustain60Minutes, unless photochemical quenching（NPQ）In20% WLExcept for a significant decrease, other parameters were not affected. Drying treatment causes significant spatial heterogeneity, with lower photosynthetic values in the upper part of the algal body compared to the middle or base, which can be clearly displayed through chlorophyll fluorescence imaging.NPQandrETRmaxThe evaluation of photosynthetic response shows high sensitivity and applicability, indicating that the impact of natural dehydration is stronger than that of high salinity or high mannitol treatment.

Beijing Yiketai is a domestic plant phenotype analysis technology enterprise dedicated to ecological analysis-Promotion, research and development, and services of agricultural health research monitoring technology, providing customers with plant phenotype analysis technology solutions:

uFP/APSeries handheld chlorophyll fluorescence analyzer

uFluorCamSeries chlorophyll fluorescence/Multispectral Fluorescence Imager

uFluorTronserieschlorophyll fluorescence/Multi functional hyperspectral imagingimaging system

uPhenoPlot®-SIFLightweight hyperspectral imaging technology

uPhenoTron® PTSPlant Phenotype Imaging Analysis System

uET-LEDIFChlorophyll fluorescence monitoringsystem

1)Pineda M, Olejníčková J, Cséfalvay L, et al. Tracking viral movement in plants by means of chlorophyll fluorescence imaging[J]. Journal of plant physiology, 2011, 168(17): 2035-2040.

2)Du G, Li X, Wang J, et al. Discrepancy in photosynthetic responses of the red alga Pyropia yezoensis to dehydration stresses under exposure to desiccation, high salinity, and high mannitol concentration[J]. Marine Life Science & Technology, 2022, 4(1): 10-17.