Congratulations to Professor Liu from Fujian Agriculture and Forestry University for establishing a simple and efficient instantaneous transformation system for "hemp" leaves

火麻(Latin scientific name:（cannabis ）yes火麻Science火麻belong toannualUpright herbaceous plant with dense branches covered in grayish white hairs; Leaves lanceolate or linear lanceolate, densely covered with grayish white flat hairs and yellow green flowers, finely covered with adherent hairsTherefore, manual injection method is difficult to introduce the bacterial solution into it.

Recently, Professor Liu Yuanyuan from Fujian Agriculture and Forestry University adopted our company'sPTS-50The model of plant live transformation system,1Within minutesSuccessfully introduced Agrobacterium intomanystrainFire hemp leavespieceTheir research has been successfully published in the latest issuePLANTSperiodical.

In this paper, the author utilizesRUBYThe reporting system is used to detect conversion results by directly observing changes in leaf color (from green to red or yellow) with the naked eye, without the need for chemical treatment or specialized detection equipment, greatly improving the convenience and efficiency of experiments. This article also discusses4Agrobacterium tumefaciens（GV3101TheEHA105TheLBA4404andAGL1）We conducted a conversion capability evaluation and ultimately found that:LBA4404andAGL1Has good conversion performance. In addition, it also adoptsGFPandGUSFurther evidence has been provided on the transformation effect of the reported genes.

The successful publication of this article provides a new, fast, efficient, and high-throughput research method for the study of gene function in hemp; This provides a possible solution for studying functional genes in other plants that are difficult to transform, such as those with wax, cilia, or other protective structures.

Author Introduction

Liu Yuanyuan, Professor and doctoral supervisor at the Strait Joint Research Institute of Fujian Agriculture and Forestry University.

Distinguished Professor of Minjiang Scholars

Selected countriesHigh end Foreign Expert Introduction Plan

Fujian Province Youth Top notch Talents Program

2015Obtained a doctoral degree from the University of British Columbia in Canada. The research group focuses on medicinal plants and conducts studies on plant active secondary metabolites. As the first author and corresponding authorPlant CellTheThe Plant JournalThePlant Biotechnology JournalThePlant Molecular BiologyWaiting for international journals to publish papers.

PTS-50Plant Living Transformation System