Anaerobic incubator

Operation Manual

Statement:

The content of this operation manual is for reference only. Please refer to the original English operation manual.

1. install

1.1 Location requirement: This instrument is intended for indoor use, with an ambient temperature requirement of15℃~30℃, must be kept away from equipment that has water vapor diffusion after heating, such as ovens, pressure cookers, etc. Avoid direct sunlight and excessive air flow﹑Heating or cooling pipelines, etc.

1.2 The instrument power supply must have good grounding, and voltage fluctuations should not exceed10%Otherwise, the instrument will not function properly.

1.3 There must be a pressure regulating device on the gas supply bottle, and the pressure is generally10psi(0.1MPA左右)

1.4 Suggested concentration is5%H₂,5%CO₂and90%N₂The mixed gas.

1.5 If you use two gas cylinders for gas supply, it is recommended that one is a mixed gas and the other is100% N_2..

Attention: In the gas entering the boxH₂The concentration cannot exceed5%

2. Control Panel

2.1 PowerControl the power supply of the entire instrument.

2.2 Set temperature:Set the temperature, the controller consists of two buttons (up/down) and four digital displays, displaying the actual temperature inside the box in degrees Celsius0.１℃

2.3 Pass box VacuumDetect the vacuum degree of the transfer chamber.

2.4 Set over tempThis protective device is an automatic temperature regulator used to monitor the temperature inside the incubator. It can prevent any malfunction of the incubator temperature controller that may cause the temperature inside the incubator to exceed the set point. If the temperature exceeds the safe temperature set point, the safety device will cut off the power supply to the heater and sound an alarm.

2.5 Air Lock Controls Manual Cycle:This manual circulation device is used to control the vacuum system and sample transfer window. pressVACAfter pressing the key, the desired mixture of gas will be filled into the air chamber, which requires 3 cycles.

2.6 Air Lock Controls Auto CycleThe automatic circulation device is used to exhaust the air chamber and inject the desired mixed gas into the chamber. This process will be carried out4Secondly or4More than once, after completion“ANAEROBIC”The indicator light will light up, and if you want to interrupt the cycle, you can keep pressing it down“START/RESET”5Just seconds.

2.7 Heating Activated:When this indicator light is on, it indicates that the main heater inside the incubator is heating up.

2.8 Over temp Activated:The indicator light is on and accompanied by an alarm sound, indicating that the safety protection device has been activated and the temperature inside the control box has been controlled by the device.

2.9 Chamber Gassing:When the light is on, it indicates that the box needs to be filled with gas.

2.10 Air Lock Doors Sealed:The light on indicates that the two doors of the sample transfer window are closed.

2.11 Gas Activated:In manual cycling, this light indicates that the gas switch is activated. In automatic tracking and

3In one cycle, the light indicates that the gas valve inside the sample transfer window is activated.

2.12 Vacuum Activated:This light indicates that the vacuum pump inside the sample transfer window is activated in manual or automatic cycling mode.

2.13 Auto Cycle Aerobic:The automatic circulation mode is not activated. When the automatic circulation mode is activated, the automatic oxygen exhaust lamp will flash.

2.14 Auto Cycle Anaerobic:When the automatic oxygen exhaust is turned on or completed, the light will turn on. When the automatic circulation device is activated, the automatic oxygen discharge indicator light will flash.

3. Connection of pipes and wires

1. The connection port on the left side of the instrument is used for gas, vacuum tube, and foot pedal control switch, and the instrument has 5 interfaces left.

3.2.Connect the pipe to the regulator of the working gas, for example, if it is an anaerobic mixture gas, it should be connected to“GAS1”On the interface.

1. Connect and exhaust oxygen gas to“GAS2”On the interface, such as nitrogen. Attention: If only one trachea and one gas cylinder are used, a T-joint should be used to connect the trachea to“GAS1 IN”and“GAS2 IN”On the intake interface, then connect the pipe to the gas cylinder.
2. Connect the instrument with a 3/8-inch tubeVAC INMouth and vacuum pump.
3. Connect the power supply of the vacuum pump. Please note that the power supply current of the pump must be higher than
4. Connect the two parts of the foot switch with a T-shaped connector.
5. Connect the foot switch tube to the left side of the instrument“GAS OUT”On the interface.
6. Connecting the vacuum tube to the instrument“VAC OUT”On the interface.
7. Connect the power supply of the vacuum pump to the reserved socket of the instrument and turn on the power switch.
8. When the power switch of the instrument is turned on, the vacuum pump will start for a few seconds and then turn off. If the pump cannot start or does not stop after starting, please refer to the manual for adjustment.
9. When the power switch of the host is turned on, the power indicator light should light up, and“Chamber Gassing” “Air Lock Doors Sealed”and“Auto Cycle Aerobic”The indicator light will also light up.

4. Barometer

4.1.Purpose: This barometer is used to measure the pressure inside the box.This barometer has the following two functions.

• It can measure the pressure inside the box in real time. If the pressure inside the box increases, the liquid level in the thin tube of the pressure gauge will decrease. By measuring the difference between the inner and outer liquid levels, the pressure inside can be determined.
• It can prevent the box pressure from being too high. If the pressure is too high, the gas will be discharged outside the box.
• Water addition steps: Use a pipette or other equipment to add distilled water to the pressure gauge, with a liquid level of 2-3 inches. The water line on the pressure gauge also needs to be filled with water, which can be injected from the top of the pressure.

5. Catalyst

5.1Catalysts are used to drive away and neutralize oxygen inside the tank, and both catalysts contain palladium particles.

1. The catalyst remains inside the box, while the other one needs to be replaced regularly
2. The chemical agent is installed on the left wall inside the box.
3. You can use it every day160High temperature activates the catalyst, with a time of approximately90Minutes to 2 hours, or can be placed before use160Activated in an oven at ℃.

6. Control temperature setting and safety temperature setting

6.1Set control temperature

To set the temperature control of the incubator, you can use the up/down button. To enter the setting mode, press the up or down button once, and the digital display will start flashing. The displayed value is the previously set temperature value. Use the up or down button to adjust the value to the desired value. If no key is pressed within 5 seconds, the instrument will display the actual temperature. Attention: At this time, the temperature safety device should be adjusted to the maximum value, that is, clockwise to the maximum value. If you want to calibrate the temperature of the incubator, you must wait for the temperature to stabilize24It will be done in hours. Section 9 of the specific method states that the box was shipped from the factory under37Corrected at ℃.

1. Full temperature setting

Wait for the temperature to stabilize at the set point24After hours, turn the temperature safety device counterclockwise until the warning light is on, then turn the knob clockwise by two small squares. It only needs to be 1 ℃ higher than the set point.

７、 温度校正

Place a standard thermometer inside the box, wait for the temperature to stabilize, and observe whether the thermometer matches the display on the digital screen. If the deviation is too large, you can simultaneously pressupandDOWNPress the button for 5 seconds, wait for two small dots to flash on the monitor, then release them, and use the key head to change the displayed value to match the thermometer reading.

8Operation

8.1 Place the samples, materials, etc. in the sample transfer window.

8.2 Close both doors inside and outside the sample transfer window.

8.3 There are two methods to activate the ventilation lock.

A. Manual

1. Place the manual gas/vacuum circulation switch in the middle position.
2. ManuallyGAS／VACSwitch toVACEnd and wait for the vacuum degree to reach17－18 in-Hg，
3. Turn the switch toGASEnd, wait“AIR LOCK VACUUM GAVUGE”The indicator light is on, about 4in-HgTurn off the gas, wait for the pressure gauge to indicate zero, that is, when the sample delivery window is at normal pressure, then turn the switch to the middle position to turn off.
4. The above program should be run at least 3 times. After all steps are completed, you can use the operation window to put the sleeve on your arm, open the inner door of the sample transfer window, move the sample and materials into the box, and follow your steps.

B. Automatic cycle

1. Confirm that the door of the sample transfer window is closed and no automatic loop program has been initiated.
2. Press“AUTO CYCLE START/RESET”Press the button to start the program“AUTO CYCLE AEROBIC / AUTO CYCLE ANAEROBIC”It will flash alternately, indicating that the program is in progress. When the program is completed“AUTO CYCLE ANAEROBIC”The indicator light will illuminate.
3. If the program cannot proceed as required in a manual loop (such as not starting, not being able to perform 3 times, not being able to open the rear door after completion, etc.), please refer to the fault prompts and troubleshoot.

Note:The automatic cycle switch cannot be continuously pressed. You can lightly press it to activate it

1. If you want to terminate the cycle, press the button continuously“AUTO CYCLE START / RESET”Switch on and off for 5 seconds, the vacuum gauge will return to the zero position, and the automatic oxygen supply and exhaust indicator lights will stop flashing.

9Enter the operation box

9.1Before putting the rubber sleeve cover on your arm, please remove all decorations on your hands, such as watches, bracelets, etc., as they may damage the rubber sleeve cover.

9.2 The sleeve must be securely worn on the arm,And there must be 4 from the cuff to the door-A distance of 6 inches.

9.3 Stepping on the foot switchVACpedal(This program is to evacuate the sleeve cover)Until the sleeve * is attached to your arm.

9.4 Stepping on the foot switchGASpedal,Open the gas and rinse the sleeves,Gas should not be injected too much,Stop when you feel a small gap between your hands and sleeves.

9.5 Repeat above9.3-9.4At least twice,To ensure that the sleeve enters the operating box in an anaerobic state or in the environment you desire.

Attention:Always keep both arms in the operating area at the same time.

Attention:During operation, the arm should not move too much or move too forcefully,Otherwise, the sleeve will detach from the connection port.

１0Exit the operation box

10.1Before exiting the operation box, the internal incubator door and transfer window door must be closed.

10.2Stepping on the foot switchGASThe pedal fills the sleeve with gas, and this process should be maintained until the arm * is removed from the control box.

10.3Put the arm window door back into the arm window and lock it securely.Please perform the following checks before removing the gloves.

Ａ、 Slowly press down on the front of the sleeve to check if there is any air leakage in both arm windows.

Ｂ、 When the front of the sleeve is pressed down, if the pressure gauge inside the box bubbles, it indicates that the door is not closed properly and there is a leakThe place needs to reopen the door for inspection“O”Circles or other suspicious places. Then repeat the stepsSuddenly 'A'. If the pressure gauge inside the box does not bubble, it indicates that the door is sealed.

10.4 Remove the sleeve and move out the arm.