CLASS II, TYPE A2

Microbial Safety Console Operating Instructions

(Biological Safety Cabinet)

type number:SG-404

SG-604

factory Card:BAKER

manufacture make Country: beauty country

1. Instrument and equipment description(System/Equipment Description)
   1. Using a microbiological safety cabinet(General)

SterilGARD ®The design of the series aims to provide mutual protection for both the operator and the operated,Also protect the environment from being contaminated by the manipulated materials and from being contaminated. The laminar flow microbial safety cabinet is suitable for biosafety levels one, two, and three(Low to moderate risk)The classification of hazard levels for the active substance is based on the standards published by the United States Centers for Disease Control and Prevention“Biosafety in Microbiological and Biomedical Laboratories” (U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and National Institutes of Health, U.S. Government Printing Office, Washington, D.C. 20402.) (HHS publication number CDC 93-8395).

Biosafety level four or hazardous substances should not be allowed to be operated in this safety cabinet,Except for asynchronous pairing with space suits(*Isolate personnel and environment,Equipped with personnel independent oxygen supply system, etc),Inside another high coverage facility.

1. Placement position in the laboratory

Proper placement in the laboratory is necessary. *The placement is in a dead corner of the laboratory,Breaking away from the movement of people like walking online,Air conditioning inlet and outlet,door,window,And other sources that can interfere with the airflow. Relevant experimental evidence,If the airflow or other interfering airflow is higher than the airflow speed at the inlet,Pollution sources may enter or leave the work area. Both can cause experimental or environmental infections.

If the exhaust of the machine is discharged indoors instead of through a pipe to the outside,It must be confirmed that there is sufficient distance between the machine and the ceiling. If the ceiling is too close to the machine exhaust,It will limit the intake air speed. Exhaust protection cover,Randomly attached inverted one”V”device,Sufficient distance can be provided. If the exhaust protection net is not installed,It must be confirmed that the distance between the machine exhaust port and the ceiling cannot be less than3' (76.2mm).

Precautions for exhaust to indoor environment:

1. Do not use the area above the machine as a storage location for items
2. Do not use flammable materials,Explosive nature,Or toxic volatile gases/vapor,Or it may lead to the former situation,Unless the safety team determines its danger. The rate filter only removes particles,Cannot filter gas.
3. Retain exhaust protection net,Do not dismantle. This net can protect the exhaust filter from items falling onto it,Maintain the spatial distance between the machine and the ceiling,The machine can only exhaust normally.
   1. Design requirements(Design Requirements)
   2. 1.This microbial safety operating platform has the following special designs:
      1. ReadySafe ModeThis feature will remain unused,Reduce total air volume and energy consumption. In this state,The fan motor of the machine automatically decelerates,But it can still maintain personnel,product,And environmental protection. start”ReadySafe”pattern,Pull down the sliding door *,close. Starting is indicated by the deceleration of the windmill motor and the automatic extinguishing of the fluorescent light”ReadySafe”pattern. When the sliding door is pulled to a safe operating height, eight”(20cm),After the windmill motor automatically returns to its original speed, it can be operated by turning on the fluorescent lamp.
      2. Adjustment of air flow balance. Equipped with built-in balance air damper adjustment to adjust the imbalance of downward airflow or intake airflow. This balance damper must be adjusted by qualified engineers and qualified instruments.
      3. The capacity of a windmill motor refers to the capacity of the windmill motor when the resistance increases(Because the filter began to adsorb particles),It can also provide a constant air volume. Confirm the resistance after the simulated filter begins to adsorb in the confirmation mode,To establishBakerAs mentioned in the specifications, it can automatically process larger than200%The pressure difference has increased,Not less than the total supply air volume10%.1/3HPThe direct drive windmill motor,1625RPM. HavingStediVOLT^RMotor speed controller(Motor Speed Controller)Automatically adjust voltage fluctuations to control the windmill/The speed of the motor,Maintain stable wind speed,Ensure a stable and continuous circulating airflow within the system to preventThe danger of airflow leakageDesign a fan motor system for *, whenHEPA FILTERWhen the airflow is insufficient due to particle blockage, the high-power motor automatically adjusts to maintain normal values±5FPMWind flow ensures the normal operation of the system and can increaseHEPA FILTERThe service life.
      4. UniPressure^TMPreflow PlenumSpecial design, made of iron for easy air tightness with the filter frame,Maintain uniform laminar air pressure, prolong the service life of the filter, and reduce airflow noise.
      5. High speed suction duct(The High Velocity Reture Air Slots)：Located on the inner side of the sliding door of the operating platform, there are designed high-speed suction slots on both sides perpendicular to the workbench, which can effectively prevent the leakage of gases, evaporated substances, and particles inside the operating platform, as well as prevent external environmental gases from entering the working area.
      6. Negative pressure pipeline:diameter3The inch opening is located on the right side inside the machine,Provide a pipeline with negative pressure enclosure,Large power cables or other experimental pipelines can be directly inserted into the control panel through pipelines. Another sealing plug,This pipeline can be sealed when not in use or smoked for disinfection.
      7. Negative pressure double-layer box structure(Negative Pressure Double-Wall Plenums)The entire work area is surrounded by negative pressure interlayers at the bottom, sides, and back, and positive pressure filtered air at the top, which can effectively prevent pollution and eliminate leaks.
      8. Air curtain design(Momentum Air Curtain)Featuring a special air curtain design, it is installed inHEPAThe stainless steel airflow distribution plate under the filter screen redistributes the filtered air flow direction. Formed close to the operator's side100 FPMThe quick air curtain is average for other work areas55 FPMNormal airflow, together with high-speed suction slots and negative pressure double-layer structure boxes, forms a safety guarantee system.
      9. Equipment structure
         1. Internal workspace: structured as a single pieceSUS304Stainless steel one-piece forming, seamless rounded corner design.
         2. External shell: forComposed of corrosion-resistant stainless steel plates,Featuring white enamel paint.
         3. Workbench: concave design for corrosion resistanceSUS304Made of stainless steel material with smooth rounded corners, the countertop does not reflect light.
         4. Operating toolsEPDMThe softness of the materialPad. This cushion is resistant to most chemical corrosion andUVto beam. Detachable, resistant to high-pressure sterilization.
         5. Negative pressure relief installationSet,When the sliding door is closed, air will be supplied from the sliding door to reduce the negative pressure burden and noise of the glass sliding door.
         6. Activity sliding door:
   3. 1/4”Thick, transparent and resistantUVSafe glass sliding door.
   4. Segmentless adjustment, can be safely pulled down or opened.
   5. Glass sliding doors are designed to conform to ergonomics10°Slope design.
   6. Alarm and mute devices with safe operating height.
      1. Lighting fixtures: The lighting tube adopts an electronic stabilizer, which can be directly started without flickering, with a comfortable eye design and a transparent outer cover.
      2. UV lamp: UV sterilization lamp, wavelength254nm.
      3. Auxiliary socket(duplex outlets)：
   7. Equipped with a waterproof protective cover.
   8. It has an independent circuit circuit that is separated from the circuits of the lighting lamp, ultraviolet lamp, and windmill motor.
   9. Equipped with residual current circuit breaker(Ground Fault Interrupter, GFI)The design.
   10. The power consumption limit for internal sockets shall not exceed5ampere! (There are additional labels inside the machine to indicate)
       1. Differential pressure gauge:Can monitor and display the positive and negative pressure difference inside the control panel.
       2. Rate filter screen(HEPA Filter)：

1. Equipped with a main filter screen(supply HEPA filter)And exhaust filter screen(exhaust HEPA filter)Both of them.
2. The filtration efficiency is99.99%effective filtration0.3umParticles.
3. The design allows for the replacement and maintenance of the filter screen to be operated from the front.
   1. Telescopic tripod:

1. Adjustable height telescopic tripod, tripod heightZui Gao is36 5/8'， Zui Low is28 1/8'.
2. include2 1/2'Adjustable balance table legs.
   1. Requirements for Public Support System
      1. Power requirements:115V ± 10%，1ψ，60Hz.
   2. safety design
      1. Four sided vertical laminar negative pressure machine, superior toCLASS II TYPE A₂Standard design.
      2. UV sterilization lamp:

1. When the windmill motor is operated, the lighting is turned on, or the glass sliding door is not *, the ultraviolet sterilization lamp cannot be started.
2. When the UV lamp is on, once the glass sliding door is opened, the UV sterilization lamp will automatically turn off.
   1. Operating control system(Operator Control System)

Equipped with microcomputer control(Microprocessor Control)Horizontal touch control panel, all button operations areOn-Offcycle mode(Pressing once isOnPress the status again forOffThe state of)As shown in Figure 1, there are a total of five buttons, which are described as follows:

1. UV lamp(UV light)button
   1. Function: It is a switch for controlling the action of ultraviolet lamps.
   2. Lamp indication: When starting, below the buttonLEDThe indicator light will light up.
   3. Safety design:

1. When the sliding door is not closed or the lighting is on,UV sterilization lamps cannot be started.
2. When the UV lamp is on, once the glass sliding door is opened, the UV sterilization lamp will automatically turn off.
   1. lighting lamp(fluorescent light)button
      1. Function: A switch for controlling the operation of lighting fixtures.
      2. Lamp indication: When starting, below the buttonLEDThe indicator light will light up.
      3. Safety design: The lighting can only be turned on when the windmill motor is running.
   2. Auxiliary socket(duplex outlets)button
      1. Function: A switch for controlling the auxiliary socket function.
      2. Lamp indication: When starting, below the buttonLEDThe indicator light will light up.
      3. Safety design: equipped withmp.Residual Current Circuit Breaker(ground fault interrupter).
   3. blower motor(Motor blower)button
      1. Function: A switch for controlling the action of the fan motor.
      2. Lamp indication: When starting, below the buttonLEDThe indicator light will light up.
   4. Alarm mute(alarm reset switch)button
      1. Function: Provides a mute effect for alarm sounds.
      2. Lamp indication: When the alarm is activated, the "SASH LEVEL''LEDThe warning light will continue to flash.
      3. Safety design:

1. When the height of the sliding door is not within8”When in a high or closed state, the safety alarm system will activate.
2. When the security alarm system is activated, pressing the 'alarm mute button' will temporarily cancel the alarm sound.
3. After pressing the 'alarm mute button',5When the unsafe state is not resolved within minutes, the alarm will sound again.

	UVGermicidal lamp		fluorescent lamp		socket		blower motor		Alarm mute

indicator light

Figure 1 Microcomputer controlled horizontal touch button operation panel

1. Security alarm system

Equipped with a safety alarm system, when the height of the movable sliding door is not at the safe height8'When the sliding door is high or not closed, the alarm system will automatically activate.

1. Alarm mode: When the alarm is activated, there will be an alarm sound and "SASH LEVELTheLEDThe warning light is flashing.
2. Silent design: Set up an "alarm mute" button on the control panel. When the alarm is activated, pressing it once can temporarily cancel the alarm sound, butSASH LEVELTheLEDThe warning light keeps flashing.
3. Safety design: The mute effect only temporarily relieves the alarm sound. If5If the unsafe condition is not resolved within minutes, the alarm will sound again
4. Programmable delay shutdown timing setting

The following steps can be used to delay the shutdown time of the program. This program setting can be used for UV lamps,socket,Or for timing with fluorescent lamps. note-The function of setting the timer must be programmable only when it is turned off.

15Minute interval setting

1. Press the function button you want to set,Not released;at about3You will hear a short sound in seconds”beep”The sound. This means that the timing program has been started,Then it represents that this feature will be available in15Automatically shut down in minutes. Release the button.
2. Continue pressing continuously,Pressing once can increase15minute.(i.e.,Pressing it three times in a row will set the automatic timer60Turn off this feature in minutes,By * individuals15Minutes plus3 x 15minute)
3. If the programmable mode exceeds4No action in seconds,Start timing now.
4. After the timer has ended,The set function will automatically turn off.
5. At any time after setting up the program,If you want to end the timer program,You can turn off the function by pressing the function key,You can also turn off the timing function.
6. When turning off the timer function or when the timer has already been completed,After the function is turned off,All programs set are reset to zero,Must be reset.

One hour interval setting

1. Press the function button you want to set,don't let go
2. at about3You will hear a short sound in seconds”beep”The sound. Continue holding down this key for approximately3You will hear a long sound in seconds“beep”The sound. This means that the timing program has been started,Then it means that this feature will automatically shut down after one hour. Release the button.
3. Continue pressing continuously,Pressing once can increase one hour.(i.e.,Pressing it three times in a row will set the automatic timer to four hours and then turn off this function,From * one hour plus3 xone hour).
4. If the programmable mode exceeds4No action in seconds,Start timing now.
5. After the timer has ended,The set function will automatically turn off.
6. At any time after setting up the program,If you want to end the timer program,You can turn off the function by pressing the function key,You can also turn off the timing function.
7. When turning off the timer function or when the timer has already been completed,After the function is turned off,All programs set are reset to zero,Must be reset.

Schematic diagram of operation instructions

Button name/icon	Method steps	observe
UV light	Confirm that the sliding door is closed. Confirm that the lighting fixtures are turned off. open(Click the button)UV lamp, observe the UV lamp andLEDIndicator light action(bright/extinguish). Close(Click the button again)UV lamp, observe the UV lamp andLEDIndicator light action(bright/extinguish).	When the UV lamp is turned on, it lights up,The UV light below the buttonLEDThe indicator light is on. When the UV lamp is turned off, it goes out,The UV light below the buttonLEDThe indicator light is off.
fluorescent light	confirmUVThe light is turned off. Click on 'Motor blowerPress the button to turn on the windmill/Motor. open(Click the button)Lighting fixtures, observe the lighting fixtures andLEDIndicator light action(bright/extinguish). Close(Click the button again)Lighting fixtures, observe the lighting fixtures andLEDIndicator light action(bright/extinguish).	When the lighting is turned on, the lighting lights up,The lighting below the buttonLEDThe indicator light is on. When the lighting is turned off, the lighting goes out,The lighting below the buttonLEDThe indicator light is off. It is necessary to confirm that the windmill motor has been started before starting the fluorescent lamp.
duplex outlets	open(Click the button)Auxiliary socket function, observeLEDIndicator light action(bright/extinguish). Test the voltage of the socket socket with a multimeter and record it. Close(Click the button again)Auxiliary socket function, observeLEDIndicator light action(bright/extinguish). Test the voltage of the socket socket with a multimeter and record it.	When activating the auxiliary socket function,Below the buttonLEDThe indicator light is on. When the auxiliary socket function is turned on, the socket voltage test is110V±10%. When the auxiliary socket function is turned off,Below the buttonLEDThe indicator light is off. When the auxiliary socket function is turned off, the socket voltage test shows low volts.
Motor blower	open(Click the button)windmill/Motor, observeLEDIndicator light action(bright/extinguish)And observe the windmill/Motor operating status. Observe the pressure difference displayed on the differential pressure gauge. Close(Click the button again)windmill/Motor, observeLEDIndicator light action(bright/extinguish)And observe the windmill/Motor operating status. Observe the pressure difference displayed on the differential pressure gauge again.	Turn on the windmill/When the motor is in operation,Below the buttonLEDThe indicator light is on and the windmill/The motor starts running. At the windmill/When the motor is running, the differential pressure gauge displays greater than0The reading value.(Suggest testing afterwards,Record pointer position) Turn off the windmill/When the motor is in operation,Below the buttonLEDThe indicator light is off and the windmill/The motor stops running. At the windmill/When the motor stops running, the differential pressure gauge displays:0.
alarm reset switch	Move the sliding door of the activity8”Position the warning sound to activate.	a.Click the button when the warning signal is activatedThe warning sound stops and the warning light continues to flash.
energy-saving function “READY SAFE” Mode	The machine is operating normallytime(The windmill motor and fluorescent lamp are both started)，Pull down the sliding door directly to * close Open the sliding door to8”Standard operating height	Windmill motor automatic reductionquick(But it continues to operate)The fluorescent light is turned off The windmill motor accelerates to normal speed. It can be operated and used by turning on the fluorescent lamp.
Safety Function Diagram
project	Method steps	safety function
UV lamp automatic Disable function	Confirm that the sliding door is closed. Confirm that the lighting fixtures are turned off. Turn on the UV light, then open the sliding door and observe the movement of the UV light and its indicator light.	When the sliding door is opened, the ultraviolet light immediately goes out, and the indicator light of the ultraviolet light also goes out.
The safety protection function of ultraviolet lamps	Open the sliding door with a height of8”following. Turn on the UV lamp again and observe the action of the UV lamp and its indicator light.	The UV light function cannot be activated, meaning that both the UV light and its indicator light cannot light up.
Lighting fixtures and windmills/Interactive function of motor	Confirm the windmill/The motor is in the off state. Turn on the lighting and observe the operation of the lighting and its indicator lights.	The lighting function cannot be turned on, that is, both the lighting and its indicator lights cannot be turned on.
Warning function for safe operation height	Open the sliding door with a height of0～8”Observe the action of the warning system within a certain height.	Warning sound sounded,The warning indicator light starts flashing.
	Open the sliding door with a height of8”Above the height, observe the action of the warning system.	Warning sound sounded,The warning indicator light starts flashing.
Silent safety function of warning system	Under the action state of the warning system(Open the sliding door more than8”The height)Press'alarm reset switchObserve the warning sound and indicator light movement. The height of the sliding door remains unchanged5After minutes, observe the warning sound and indicator light action again.	Press'alarm reset switchAfter that, the warning sound immediately stopped, but the warning indicator light continued to flash. 5After minutes, the warning sound sounded again and the warning indicator light continued to flash.