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E-mail
zhongsilifu@163.com
- Phone
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Address
Intersection of Sanshui Road and Fenglin Road, Hexi District, Tianjin
Zhongsilifu (Tianjin) Biotechnology Co., Ltd
zhongsilifu@163.com
Intersection of Sanshui Road and Fenglin Road, Hexi District, Tianjin
| ingredient | Function and Function | Recommended ratio | Precautions |
| Fresh royal jelly | Main raw materials, providing active ingredients（10 -hda.Protein, vitamins) | 80%~90% | Fresh collection is required (acidity)≤53 mL/1N NaOH） |
| trehalose | Low temperature protectant, maintains protein structure and enzyme activity | 5%~10% | Compound with mannitol can increase the glass transition temperature |
| maltodextrin | Filler, improve the structure of freeze-dried cake, prevent collapse | 3%~5% | DEvalue10~15Avoid moisture absorption |
| ascorbic acid | Antioxidants, inhibit lipid oxidation and color deterioration | 0.1%~0.3% | With citric acid（0.05%）Collaborative efficiency enhancement |
| lecithin | Natural emulsifiers enhance solubility and dispersibility | 0.5%~1% | Avoid contact with metal ions to prevent degradation |
orthogonal experimental design: With10 -hda.Select the optimal ratio based on retention rate and appearance of freeze-dried cake (without collapse or cracking).
Accelerated stability testing：40℃/75% RHStorage under conditions3Month by month, detect changes in active ingredients（HPLCThe law).
Design of freeze-drying process curve for royal jelly
Quick freezing strategy：
pre-freezing temperature：-45℃Ensure vitrification below.
降温速率：1℃/min(Avoid large ice crystals damaging cell structure).
annealing treatment: In-25℃insulation2Hour by hour, optimize ice crystal distribution.
| parameter | set value | key control points |
| 隔板温度 | -40℃ →-10℃(Gradient heating) | heating rate0.3℃/hTo avoid exceeding the collapse temperature（Tc） |
| 真空度 | 50~100 Pa | Cooperate with the temperature of the cold trap（≤-55℃）Ensure efficient sublimation |
| duration | 20~24hour | Pressure Rise Test（ΔP＜5 Pa/30min）Determine the endpoint |
| parameter | set value | key control points |
| 隔板温度 | 25℃→35℃(Gradient heating) | heating rate0.2℃/minTo prevent local overheating |
| 真空度 | 10~30 Pa | Maintain high vacuum to promote desorption of bound water |
| duration | 6~8hour | Residual moisture≤3%(Karl Fischer method)
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