1. According to national standards and relevant regulations, explain the use and limitations of the biosafety cabinet to all users who may use it. Written regulations, safety manuals, or operation manuals should be provided to the staff. Especially, it is necessary to clearly inform the staff that in the event of splashing, breakage, or improper methods, the safety cabinet cannot protect the operators.

2. The safety cabinet can only be used when it is working normally.

The glass observation window on the front face of the safety cabinet shall not be opened when in use at the safety cabinet.

4. The instruments and materials placed inside the cabinet must be kept in low quantities. The air circulation at the rear of the static pressure box must not be obstructed. Before placing the materials in the work area of the safety cabinet, their surface should be cleaned.

5. Bensheng lamps (a type of gas lamp) are not allowed to be used inside the cabinet, as the heat they generate can interfere with the airflow and may damage the filter. Micro electric incinerators can be used, but using disposable sterilization rings is better.

6. All work inside the cabinet should be carried out in the middle or rear of the working face and visible from the observation panel.

7. The movement behind the operator should be minimized.

8. The operator should not extend their arms back and forth into the cabinet to avoid interfering with the airflow.

9. The front delivery style grille must not be blocked by notes, droppers, or other materials, otherwise it may interfere with the airflow and potentially contaminate the materials, leaving personnel exposed.

10. The surface of the safety cabinet should be cleaned with a suitable disinfectant after each task is completed and at the end of the day.

11. The fan of the cabinet should run for another 5 minutes before and after the work is completed.

Location of biological safety cabinet placement

The airflow velocity entering the biosafety cabinet from the front face opening is approximately 0.45 meters per second. At this wind speed, the consistency of one-way flow inside the cabinet is easily disrupted by the movement of personnel near the safety cabinet, opening windows, supplying air and distributing fans, and opening and closing doors. According to the ideal layout, it should be placed away from activities and areas that may interfere with airflow. Try to leave a space of 30 centimeters behind and on both sides of the safety cabinet for maintenance operations. Leave a height of 30-35 centimeters above the cabinet to measure the wind speed of the exhaust filter and leave enough space for the replacement of the exhaust filter.

Cleaning and disinfection of biosafety cabinets

All items inside the biosafety cabinet, including equipment, should undergo surface cleaning treatment and be removed from the cabinet after the work is completed, as residual culture media may affect the growth conditions of microorganisms. The inner surface of the safety cabinet should be cleaned before and after each use. To wipe the surface and inner wall of the workbench with disinfectant, the disinfectant used should be able to kill all microorganisms that may exist inside the cabinet. At the end of the day's work, the back surface should be cleaned by wiping the workbench, all sides, back, and inside of the glass. Bleach solution or 70% alcohol can be used, which are effective disinfectants for target organisms. If using bleach solution, a corrosive disinfectant, it is necessary to wipe it again with sterilized water. It is recommended that the work cabinet be in working condition during cleaning and disinfection. If it is not in working condition, it should run for 5 minutes to clear the gas inside the safety cabinet before it is closed.

Decontamination treatment of biosafety cabinets

The decontamination of Level 1 and Level 2 biosafety cabinets involves placing an appropriate amount of formaldehyde oligomers (to achieve a final concentration of 80% formaldehyde oligomers in the air inside the cabinet) on an electric heating plate or frying pan (controlled from outside the cabinet). Also place another electric heating plate or frying pan containing 10% more hydrogen carbonate than paraformaldehyde inside the cabinet (controlled outside the cabinet). This second container should come with a lid that can be removed from a distance (e.g. tied to a wire that can be pulled from outside the cabinet). This can reduce the amount of oligomeric formaldehyde that is neutralized in advance. If the relative temperature is below 70%, there is an open container with hot water. If there is no front partition, a bioplastic baffle should be used and taped to the front face to ensure that no gas leaks into the room. When all the formaldehyde oligomers in the tray have evaporated or the switch has been turned on for 1 hour, turn off the switch. Leave the safety cabinet untouched throughout the night. Then remove the lid of the second plate and turn on its switch, and the bicarbonate begins to evaporate. At this point, turn off the switch on the plate and let the safety cabinet start working, allowing the bicarbonate ammonia gas to circulate for 1 hour. Then the front partition (or plastic baffle) can be removed, and the safety cabinet can be used.

Zhejiang Sujing Purification Equipment Co., LtdLocated in Daoxu Town, Shangyu District, Shaoxing City, Zhejiang Province, it is one of the manufacturers in the purification equipment industry in East China. We can provide air purification system engineering design, construction, testing, and technical services for industries such as microelectronics, biomedicine, hospital operating rooms, fiber optic cables, food and beverage, precision instruments, semiconductors, and new material applications in accordance with ISO14644-1 standards, GB50073-2001 national standards, and national GMP specifications.
The company's main products include purification workbench series, air shower room series, fume hood series, and biosafety cabinet seriesWidely used in healthcare, electronics, pharmaceuticals, biology, food, agriculture and forestry, animal husbandry and veterinary medicine, inspection and quarantine, aerospace, automotive manufacturing, precision instruments, colleges and universities, and various research institutions, it enjoys a high reputation in the Southeast Asian market.
Zhejiang Sujing Purification Equipment Co., Ltd. is dedicated to serving consumers and distributors with our products; Humanized services; Low cost; We warmly welcome your joining!