Desktop microcentrifuge is a laboratory equipment designed specifically for processing small volume samples. It has the characteristics of small size, easy operation, and stable performance, and is widely used in biomedical, clinical laboratory, molecular biology, pharmaceutical and other fields. The following is a detailed introduction to its core functions and uses:
1、 Core functions
Micro sample centrifugation
Capacity range: Typically equipped with 0.2mL to 2.0mL microcentrifuge tube adapters, it can handle small volume samples such as PCR tubes, EP tubes, and microplates.
Rotational speed and centrifugal force: The maximum rotational speed can reach 15000-20000rpm, and the centrifugal force (RCF) range is 10000-30000 × g, meeting the requirements for rapid separation.
Short time centrifugation: supports high-speed centrifugation for a short period of time (such as 30 seconds to 5 minutes) to quickly precipitate particles or separate liquid phases.
Multi mode operation
Timer function: The centrifugation time can be preset (1 second to 99 minutes), and a prompt sound will be emitted after automatic stop.
Speed/centrifugal force switching: Supports switching between rpm (revolutions per minute) and RCF (relative centrifugal force) units to meet different experimental needs.
Instantaneous centrifugation (Pulse): Rapid mixing or precipitation is achieved through brief acceleration, avoiding excessive centrifugation.
safety protection
Automatic balance detection: Equipped with an imbalance sensor, it automatically detects sample distribution before centrifugation to prevent equipment damage caused by unbalanced load.
Door lock protection: The door cover automatically locks during operation to prevent accidental opening.
Overspeed protection: Automatically shuts down when the speed exceeds the set value to avoid sample splashing or equipment failure.
2、 Main purpose
Molecular Biology Experiment
DNA/RNA extraction: Quickly precipitate cell debris, proteins, or salt ions to purify nucleic acid samples.
Preparation of PCR reaction system: Centrifuge the mixed reaction solution, eliminate bubbles, and ensure uniform distribution of reagents.
Enzymatic cleavage reaction: Separate enzymatic cleavage products from unreacted DNA fragments.
Clinical testing and diagnosis
Blood sample processing: Separate plasma/serum from blood cells for biochemical testing or immunoassay.
Urine/body fluid analysis: Concentrate cells or microorganisms in the sample to improve detection sensitivity.
Rapid diagnostic kit: Combined with microfluidic chips, it achieves rapid separation and detection of samples.
Pharmaceutical and Biotechnology
Drug screening: Centrifugal separation of metabolites or secreted proteins from cell culture supernatant.
Protein purification: Combined with affinity chromatography technology, rapidly precipitate the target protein.
Nanoparticle preparation: Separate nanoparticles from unreacted raw materials and control particle size distribution.
Environmental and Food Testing
Microbial detection: Concentrate bacteria or viruses in water or food samples.
Heavy metal analysis: Separate solid particles from liquid phase in the sample for subsequent atomic absorption spectroscopy detection.
3、 Technical advantages
High Efficiency and Energy Saving
Adopting a brushless DC motor, it starts quickly, has low noise (usually ≤ 55dB), and consumes only one-third of the energy of traditional centrifuges.
Compact design (size approximately 30cm x 40cm x 25cm), saving laboratory space.
precise control
The digital display screen displays real-time parameters such as rotational speed, centrifugal force, and time, with an error of ≤± 1%.
Support program storage function, which can save commonly used centrifugation conditions (such as DNA extraction mode).
Strong compatibility
It can be adapted to various specifications of centrifuge tubes (such as 0.5mL, 1.5mL, 2.0mL), and some models support microplate centrifugation.
Equipped with a rotor recognition system, it automatically matches the optimal centrifugal parameters to prevent misoperation.
4、 Typical application scenarios
PCR Laboratory
Centrifuge and mix the reaction solution in an 8-tube or 96 well plate to ensure sufficient contact between the primer and the template.
Quickly precipitate impurities in PCR products to improve subsequent sequencing or cloning efficiency.
Cell Culture Room
Separate extracellular vesicles or viral particles from the supernatant of cell culture.
Concentrate low concentration cell samples for flow cytometry analysis.
on-site testing
Portable design (some models weigh ≤ 5kg), suitable for field sampling or bedside testing (POCT).
Combined with a rapid detection kit, achieve real-time separation and analysis of samples.
5、 Selection suggestions
Select based on sample type
Nucleic acid/protein samples: Priority should be given to high-speed (≥ 15000rpm) models to ensure sufficient precipitation.
Cell/Microbial Samples: Choose models with low-temperature cooling function to prevent sample overheating.
Select based on flux demand
Low throughput: 12-24 tubes per treatment, suitable for personal laboratories.
High throughput: Single processing of 48-96 tubes, suitable for core laboratory or industrial applications.
Select based on budget
Basic type: priced at approximately 5000-10000 yuan, meeting the needs of conventional centrifugation.
High end model: priced at approximately 15000-30000 yuan, with advanced features such as program control and low-temperature cooling.