The efficiency and product quality of the fermentation process are influenced by various factors, among which the selection of feeding strategy is particularly crucial, as it is the key to ensuring the stable progress of the biological reaction process. Supplementing not only needs to meet the nutritional requirements for microbial growth, but also adapt to strict control of environmental conditions during the production process.

This article will delve into the feeding bottle for fermentation tanksThe precise control of the connection method and feeding time is aimed at providing practical guidance for optimizing the fermentation process.

1、 How to connect the feeding bottle of the fermentation tank?

Connection of replenishment bottle: 16 # silicone tube inside the bottleUsing a gravity ballConnect to the bottom of the bottle.

Two way connector on the lid: Connect the end with a gravity ball using a suitable length of silicone tube and use a peristaltic pumpReceive the filling interface on the tank surface; Connect a filter to the other end using a silicone tube. The overall layout is as follows:

2、 Operation in material replenishment connection

1. If conditions permit, we recommend placing the glass fermentation tank and feeding bottle together in a sterilization pot for high-temperature sterilization treatment to effectively prevent microbial contamination.

When performing this step, it is important to pay attention to the fixation of the silicone tube: use a hose clamp to tightly clamp the silicone tube connecting the glass jar and the feeding bottle. This preventive measure is crucial because it can prevent the mixing of the culture medium in the two containers due to pressure changes during the sterilization process, thereby ensuring the aseptic state of the entire fermentation system.

2. If conditions do not permit, such as inability to sterilize with ammonia water, inconsistent sterilization conditions between fermentation medium and supplementary medium, and inability to meet sterilization pot capacity, glass jars and supplementary bottles need to be sterilized separately. Therefore, Ruhr one-way valves need to be connected at both ends of their connectionTo avoid bacterial contamination caused by connecting pipelines.

Only the above two methods are recommended for connection. If neither of the above conditions can be met, it is recommended to connect the glass jar and replenishment bottle separately in the ultra clean table after sterilization is completed.

3、 Regarding the feeding time for fermentation tanks

1. Growth stage

Generally, it is during the growth stage of microorganisms, especially during the exponential growth period, when there is a greater demand for nutrients. Adequate feeding can promote rapid microbial reproduction.

During the stable fermentation period, the metabolic activity of microorganisms gradually shifts towards product synthesis. It may be necessary to accurately add relevant precursor substances or inducers based on the synthesis pathway and metabolic regulation mechanism of the product to improve the yield and quality of the product.

In the early stage, which is the lag period, microorganisms need to adapt to the new environment and have slow metabolism, so feeding is generally not necessary. In the late stage of fermentation, which is the decline period, large-scale feeding is no longer carried out unless it is to maintain the stability of the product or promote the conversion of remaining substrates.

2. At the same time, it is necessary to supplement according to the changes in the main parameters during the fermentation process:

① PH value: When microbial metabolism causes significant changes in the pH value of fermentation broth, it can be directly adjusted by acid-base solution or by using organic substances with appropriate carbon nitrogen ratios.

② Dissolved oxygen value: The sudden decrease in dissolved oxygen indicates active microbial metabolism and accelerated nutrient consumption, at which point feeding can be carried out.

Our equipment has corresponding associated functions for both of these feeding methods, which can meet the needs.

③ Extended functions:

By analyzing the changes in components such as carbon dioxide and oxygen in exhaust gas, the metabolic status of microorganisms can be indirectly reflected, providing reference for the selection of feeding time.

4、 Xinshun Fermentation TankThe replenishment strategy

In addition to the feeding methods based on parameter changes mentioned above, the Xinshun Glass Fermentation Tank series also includes manual feedingTimely replenishment of materialsEquation replenishmentIndex replenishmentFour basic replenishment strategies.

1. Manual replenishment

Set the speed and feeding amount of the manual feeding pump. After starting, the system can calculate the required working time of the pump under constant speed and quantity conditions, as well as display the remaining working time of the feeding pump. After setting the replenishment amount, the pump stops working.

2. Timely replenishment of materials

By inputting the required unit time replenishment amount, replenishment pump speed, and replenishment pump working time, the control cycle of the replenishment pump can be obtained, and periodic timely and quantitative replenishment settings can be made.

3. Equation replenishment

Enter the values of key parameters a and b in the equation feeding, as well as the required running time t for equation feeding, and start the correlation to perform equation feeding.

4. Index replenishment

Enter the values of key parameters F1 (0) and μ in the index replenishment, as well as the required running time t for index replenishment, and start the correlation to perform index replenishment.

In the actual fermentation process, it is necessary to comprehensively consider various factors and combine them with specific fermentation processes and production requirements to select appropriate feeding time and feeding strategies.

Accurately selecting the feeding time during the fermentation process is a complex and critical task that requires a deep understanding of the physiological characteristics of microorganisms, the characteristics of fermentation processes, and the application of various monitoring and control technologies. Reasonable feeding time selection can significantly improve fermentation efficiency and product quality, bringing greater economic benefits.