《GB 31659.10-2025 National Food Safety Standard - Determination of β - lactam residues in poultry eggs - Liquid chromatography tandem mass spectrometry methodIt is an important food safety testing standard jointly issued by the Ministry of Agriculture and Rural Affairs, the National Health Commission, and the State Administration for Market RegulationOfficially released on June 3, 2025And inImplemented from September 1st, 2025.

1、 Scope of application and detection objects

（1） Scope of application

Mainly targetingPoultry and egg productsIncluding common edible poultry eggs such as eggs, duck eggs, goose eggs, etc.

（2） Detection object

The specific drugs tested in this standard include the following 7 types of beta lactam drugs:

1. Penicillins:Including penicillin V, penicillin G, ampicillin, chloramphenicol, amoxicillin

2. Cephalosporins:Including cephalosporin and cefuroxime

2、 Operation process

(1)sample preparation

1. Sampling homogenization:Shell the poultry eggs and stir thoroughly with a homogenizer;

2. Cryopreservation:Freeze the homogenized sample below -18 ℃ to avoid repeated freezingRong.

(II)Sample extraction

1. Weighing and labeling:Accurately weigh about 2 grams of homogeneous sample and add isotopic internal standard working solution;

2. Solvent extraction:Add extraction solvent (such as acetonitrile solution containing formic acid), vortex and mix well;

3. Oscillatory centrifugation:Shake vigorously for 10-20 minutes, then centrifuge at 4 ℃ and above 8000r/min for 5-10 minutes;

4. Collect the supernatant:Take the supernatant and transfer it to a clean container.

(3)Sample purification

1. Dispersed purificationAdd dispersed solid-phase extraction fillers (such as C18, PSA, etc.) to the supernatant;

2. Vortex centrifugationAfter 1-2 minutes of intense vortexing, centrifuge again under the same conditions;

3. Constant volume filtrationTake the supernatant, dilute it with the initial mobile phase, and filter it through a 0.22 μ m filter membrane into an injection bottle for testing.

(IV)Determination by liquid chromatography tandem mass spectrometry

1.Liquid chromatography conditions：

（1）chromatographic column：C18 column.

（2）mobile phase：A is a 0.1% formic acid aqueous solution, and B is acetonitrile.

（3）Elution method：Adopting gradient elution.

（4）column temperature：40℃。

2.Mass spectrometry conditions：

（1）ion source：Electric spray ion source.

（2）scanning mode：Positive ion mode.

（3）Detection Mode：Multi reaction monitoring.

3.quantitative analysis：

Using the internal standard method, quantitative analysis of the target drug is performed by drawing a standard curve.

3、 Summary

β - lactam drugs are a class of antibiotics widely used in animal husbandry, including penicillins and cephalosporins, for the prevention and treatment of bacterial infections. However, improper use may lead to drug residues in poultry eggs, which can then enter the human body through the food chain, potentially threatening consumer health and potentiallyInducing bacterial resistanceAs a manufacturer of standard materials, Xibiao provides assistanceGB 31657.8-2025Welcome all major customers to come and purchase!