01 Knowledge background

Do you really understand the culture medium?

Basic culture medium plays a crucial role in organoid culture, and it also needs to be slightly adjusted according to the organoid culture needs of different organ tissue sources. In most organoid cultures, such as gastrointestinal organoids, Advanced DMEM/F-12 is usually chosen as the base medium. However, some literature reports that RPMI-1640 medium is used in the construction of lung organoids, while DMEM medium is used in the construction of brain organoids. This diversity reflects the highly customized nature of organoid culture, providing flexibility for simulating the characteristics of different organ tissues. Therefore, when selecting the basic culture medium, it is necessary to adjust and optimize it according to the specific requirements of the research and the characteristics of the constructed organoids.

Is phenol red necessary in the culture medium?

Phenol red is a commonly used indicator, usually added to the culture medium to determine its acidity and alkalinity, as well as to observe cell growth. The culture medium containing phenol red appears yellow at pH 6.8 and below. When the pH reaches around 7.6, the solution appears pink, and when the pH is above 8.2, it appears purple red. By observing the color change of phenol red, it is convenient for us to determine the pH value of the culture medium.

However, not all cases require the use of media containing phenol red. Phenol red is not an essential component in the culture medium. Many foreign vaccine or antibody manufacturers use phenol red free culture medium in the production process, and the presence of phenol red may even interfere with experimental results. Research has shown that phenol red may have estrogen like activity, meaning that in vitro or in vivo models, it can bind to estrogen receptors and trigger some estrogen reactions, seriously affecting the accuracy and reliability of experimental results. Usually, when we choose matrix gel for organoid culture, we tend to prefer low factor matrix gel without phenol red. However, the existing organoid culture media on the market all contain phenol red, so the role of non phenol red matrix gel is meaningless.

What can model-based organisms bring to everyone?

Based on the above issues, our company has developed a specialized basic culture medium OC-Advanced DMEM/F-12 (Phenol Red Free) for phenol red organoids, which helps researchers obtain more reliable experimental results. This product can be paired with bio based low factor matrix glue (082703) to create your organoid culture combination. Phenol free red, high efficiency, high quality. The future without phenol red has arrived, take action now! Don't miss out on this revolution in scientific research. Your organoid culture has never been so outstanding!

02 Product advantages

03 New Product Information

Part 04 Product Data Testing

05 Product Instructions

organoidCulture medium preparation

• Remove the OC-Advanced-DMEM/F-12 specialized basic culture medium for modeling biological organs from the refrigerator at 4 ° C and restore it to room temperature;
  

• Add 2% O27 medium additive (MB-23061A010, 50X) to the specialized basic culture medium for model based biological organs, so that the final concentration is 1X (for example, 10mL of O27 needs to be added for every 500mL of basic culture medium);
  

• Add different cytokines, small molecule inhibitors, amino acids, hormones, and so on according to the different types of organoids cultured;
  

• After the preparation of organoid culture medium is completed, it can be stably stored in a 4 ° C refrigerator for up to one week. It is recommended to store it at -20 ° C after packaging.

06 Model Classroom

Does phenol red have an impact on the culture of cells or organoids?

Research has shown that phenol red has estrogen like functions, meaning that in vitro or in vivo models, it can bind to estrogen receptors and trigger some estrogen responses. In some cell lines (such as breast cancer cells), phenol red can not only promote the proliferation of breast cancer cells, but also promote the expression of estrogen receptor related genes and the activation of estrogen metabolism and signal transduction related pathways in breast cancer cells. In addition, phenol red may cause intracellular sodium/potassium imbalance in serum-free medium, affecting cell growth.

Under what circumstances should phenol red free culture medium be used?

Phenol red is a commonly used pH indicator, usually added to cell culture media to determine the acid-base properties of the medium and observe cell growth. However, some gonadal related cell or organoid cultures may need to avoid using phenol red as it can interfere with experimental results, especially in studies related to estrogen. This is because phenol red itself has a certain estrogenic activity, which may interfere with the estrogenic response of gonadal cells or organoids. Therefore, we recommend using phenol red free culture medium when cultivating cells or organoids related to the gonads.

How can I determine if the nutrient content in the culture medium is sufficient without phenol red?

Organoid culture medium not only provides nutrients for cell growth, but also various cytokines required for organoid growth and differentiation. Therefore, we need to replace the culture medium in a timely manner when cultivating organoids. We usually recommend changing the culture medium every 3 days, which makes the role of phenol red as an acid-base indicator redundant, and phenol red does not have any nutritional components or supportive functions that affect cell growth. Therefore, the presence of phenol red is not necessary in organoid culture.

Recommendations for other products of 07 Model Biology

Model based organism O27 is an optimized organoid culture additive designed to support the cultivation of organoids. After multiple organ and neuron culture evaluations, O27 not only has the advantages of traditional B-27, but also incorporates customized factors for different organoid cultures, thereby achieving a more accurate and stable organoid and cell culture environment. It will help researchers better simulate the function of human organs, promote scientific progress and medical innovation.

The digestion solution of organoids derived from various mammals (such as humans, mice, pigs, bats, cows, etc.) can be used for routine passage of organoids. It can separate organoids from the matrix gel and gently digest them into small cell clusters or single cells, while maintaining their growth vitality after passage.

Model based biological epithelial organ basic culture medium is an optimized basic culture medium used for mammalian epithelial organ culture. The basic culture medium for epithelial organs needs to be supplemented with serum substitutes, usually O27 additives and growth factors, as this medium does not contain proteins or any other animal derived components.

Model based biological organ culture moisturizing solution is a consumable solution for cell culture to prevent cell adhesion. It can be used to lubricate various specifications of centrifuge tubes, culture dishes, culture plates, pipettes, filters, etc. to reduce their surface tension and reduce cell adhesion. Especially in the process of organoid culture passage and cryopreservation, it can effectively avoid the problem of sample loss caused by cell adhesion.

Model based biological organ cryopreservation solution can be used for low-temperature long-term storage of various mammalian organs and cell lines (such as humans, mice, pigs, bats, cows, etc.). The cryopreservation solution does not contain serum or any animal derived components, but contains 10% DMSO.

08 About Modular Biology

Xiamen Mogengel Biotechnology Co., Ltd. is located in Xiamen, China and is an enterprise that provides comprehensive solutions for organoid culture; We are a high-tech enterprise that integrates research and development, intelligent manufacturing, sales, and service, dedicated to the research and industrialization upgrading of 3D organoid culture materials.

The company has four major technology platforms, including a protein expression and purification center, an experimental animal center, an organoid research center, and an intelligent microscopic observation research center. Received honors such as Xiamen's "Double Hundred Talents" enterprise and Taicang's "First Prize in the International Youth Entrepreneurship Competition". At present, the company has products such as matrix glue, organoid culture medium, cytokines, organoid culture consumables, and intelligent microscopes. More than 40 research teams have used our products to publish articles, and our customers are all over the world.