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Wuxi Yuanqing Tianmu Biotechnology Co., Ltd
Date: 2025-05-27Read: 1
Introduction:
Highlights of this study
The gold algae mutant A4-1, which contains 43.8% protein, was combined with SPI in a mass ratio of 5% DAP+95% SPI during high water extrusion (HME) using wall breaking algae powder (DAP)
texture：The elasticity of the extruded material increased by 210.6%, the chewiness increased by 141.7%, and the hardness decreased by 19.36%.
structure：The three-dimensional structure is rich in fibers, with a significant increase in the proportion of alpha helices in the secondary structure and a significant increase in the bound water content.
digestion：In vitro simulated digestibility significantly increased by 37.6%, while reducing the bitterness and astringency of the extruded material.
Background of bacterial strains
Mutant A4-1: Induced by atmospheric pressure room temperature plasma (ARTP) mutagenesis technique from the wild typeAuxenochlorella pyrenoidosaThe golden mutant strain selected from the screening.
Core advantages: Compared with the green wild-type (WT) strain, the A4-1 mutant in dark culture appears yellow, with a 118 fold reduction in chlorophyll a and no detection of chlorophyll b. The protein (44.22% DW), total amino acids (AAs, 34.84% DW), and essential AA (17.50% DW) content are high, with increases of 31%, 22%, and 30%, respectively, compared to WT (p<0.05).
Technical Highlights:Biological Cultivation Process Online Detector (BODS)
Fully automatic intelligent fermentation
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Fermentation conditions
The initial cell density is 1 × 10 ^ 8 cells/mL; pH value is 6.5, controlled by adding 1.0 mol/L nitric acid and supplementing nitrogen source; Temperature 30 ℃; Mixing speed 80 r/min; The initial ventilation rate is 1.5 L/min. Under the same conditions, parallel fermentation tests were conducted using ammonium chloride (1.52 g/L) or urea (1.1 g/L) as nitrogen sources, with the same nitrogen molar concentration as the fermentation using sodium nitrate as the nitrogen source mentioned above.
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process monitoring
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real-time monitoring
As shown in Figures 1A and B, cell density and biomass concentration continue to increase and enter a stable phase at approximately 72 to 84 hours.
By automatically detecting and controlling the replenishment through BODS,Control the glucose concentration during the process according to the process, and maintain the nitrogen concentration within the range of 494 ± 53 mg/L through feeding (Figure 1D).
As shown in Figure 1E, when ammonium chloride is used as the nitrogen source, the protein content (dry weight 43.3%) and productivity (1.31 g/L/day) are high, while the carbohydrate content (dry weight 24.1%) and total lipid content (dry weight 13.8%) are low.
Core discovery: How microalgae powder reshapes plant meat
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Structural upgrade
Microscopic 3D network: Scanning electron microscopy shows that 5% DAP forms animal like muscle fiber structures and uniform pores in the extruded material.
Moisture locking: Low field nuclear magnetic resonance (LF-NMR) confirmed that the addition of DAP increases the proportion of bound water and enhances the water retention of the product. The addition of 5% DAP showed the highest fixed water ratio (83.70%). The increase in bound water is usually negatively correlated with water activity, thereby inhibiting microbial growth. The free water content has decreased by 62%, reducing water loss.
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Flavor optimization
Innovation and Prospects
This study introduces a new strategy that utilizes wall breaking algae powder (DAP) from the chlorophyll synthesis deficient protein nuclear microalgae mutant (strain A4-1), which not only eliminates the green and odor limitations of traditional algal biomass, but also synergistically enhances the structural integrity and bioavailability of SPI based meat analogues under high water compression conditions. DAP helps to rearrange the polypeptide chain of protein during extrusion cooling, significantly improving the elasticity of 210.6%. The increase in the proportion of bound water also enhances the stability of protein gel, and promotes the formation of protein fiber, which is a breakthrough in the quality optimization of vegetable based meat products.
In the future, we can continue to explore the fermentation performance of the green algae defect mutant (A4-1 strain) in large-scale microalgae fermentation production under industrial conditions, as well as the performance of ammonium chloride in protein synthesis, and the specific mechanism of algal components in regulating protein macromolecule interactions.
Regarding BODS
The Bioreaction Online Detection System (BODS) is an instrument that performs fully automatic online sampling, processing, detection, and sample retention of bioreactors. Multiple detection modules are integrated to display real-time biomass, substrate consumption, and product generation in the tank. The feedback control system can also be adjusted in a timely manner to achieve precise control of substrate flow, improve fermentation process control efficiency, and provide strong data support for fermentation process optimization and process amplification.
About Us
Tianmu Biotechnology focuses on the development and application of instruments and equipment in the field of biological breedingWe strive to transform and enhance the traditional strain development model of the industry through efficient mutation technology and high-throughput screening technology, improve the quality and efficiency of the biomanufacturing industry, and enhance the core competitiveness of China's biotechnology industry.Committed to providing excellent solutions for industry challenges such as low efficiency in cell and strain development and screening, expensive equipment costs, and difficulties in industrialization landing.
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