When operating the dual wave all-around electroporation instrument, the following key points should be noted to ensure the normal operation of the equipment and the safety and effectiveness of the experiment:
Preparation before operation:
Clean the skin: Before using the electroporation device, clean the skin of the operating area to ensure that there is no dirt, cosmetic residue, or oil, and to avoid infection or skin irritation when inserting the needle.
Instrument inspection: Check whether the instrument is clean, especially the needle part, to ensure that there is no wear, looseness or other problems. If necessary, replace the needle in a timely manner.
Parameter setting: Set appropriate parameters based on experimental requirements and cell types, such as voltage, pulse width, and pulse frequency. These parameters directly affect the effectiveness of electroporation and cell survival rate.
Operation process:
Insert needle: Carefully insert the needle into the skin, ensuring the insertion depth is appropriate and avoiding being too deep or too shallow.
Stable operation: During the operation, maintain stable and uniform pressure to ensure tight contact between the needle and the cell membrane.
Observing cell status: Pay attention to observing cell status, adjust parameters or stop operations in a timely manner to avoid excessive damage or death to cells.
Safety and Protection:
Wear protective equipment: Operators need to wear personal protective equipment such as protective goggles and gloves to ensure safe operation.
Adhere to operating procedures: Strictly follow safety operating procedures to avoid safety accidents caused by illegal operations.
Environmental requirements: Ensure a clean and orderly operating environment, avoiding the accumulation of debris. The equipment should be placed on a stable tabletop, and the shock absorber should be kept level.
Subsequent processing:
Clean the skin: After the electroporation is completed, carefully clean the skin of the operating area.
Cell culture: Ensure that cells are in a suitable growth environment for subsequent experiments or treatments.
Equipment maintenance and upkeep:
Regular inspection: Regularly check whether the various safety devices of the equipment are working properly, and promptly identify and address any safety hazards in the equipment.
Cleaning and maintenance: Regularly clean the surface and internal components of the equipment to keep it clean.
Record management: Establish detailed maintenance logs to record the date, content, identified issues, and measures taken for each maintenance.