Tianjin Lanbo post derivatization technology and its application in drug and food analysis

Post column derivatization technology is a commonly used online processing technique in analytical chemistry. Post column derivatization technology has opened up broad application prospects for the analysis of trace drugs and weak UV absorbing substances in media, and has been developed to improve traditional methodsHPLC(Liquid Chromatography) A commonly used technique for testing proficiency. It has a wide range of applications in the fields of pesticide residue detection, amino acid analysis, inorganic ion analysis, biotoxin detection, veterinary drug analysis, and in vivo drug analysis in traditional Chinese medicine and food.

Tianjin Lanbo has nearly10 We have collected and organized the application and maintenance experience of post column derivatization systems, as well as typical post column derivatization reactions widely used domestically and internationally, and their applications in drug and food analysis. We have also looked forward to the application prospects of new technologies in the field of drug and food analysis.

At present, the derivative system of Tianjin Lanbo Pillar is mainly mainstreamAgilentThermoShimazuWatersWe provide users with stable and reliable online derivatizers for use with imported brands of liquid chromatographs.

Derivative technology is the use of derivative reactions to generate derivatives between the analyte and corresponding derivative reagents, in order to change the chemical and physical properties of the analyte and enable its separation or detection. Derivative technology can be divided into pre column derivatization and post column derivatization2 Class. Pre column and post column derivatization techniques are widely used in the detection of trace components in weak UV absorbing substances and biological matrices. Compared with post column derivatization technology, pre column derivatization technology has shortcomings such as long reaction time, cumbersome operation steps, poor reproducibility, and difficulty in continuous and automated analysis and determination. Therefore, pre column derivatization technology is gradually being replaced by post column derivatization technology.

1.Application of post column derivatization technology in drug analysis and clinical pharmaceutical research

Analysis of aminoglycoside antibiotics

The analysis of carbohydrate substances, among which the analysis of voglibose is the most common.

Analysis of endogenous metabolites

2. Application of post column derivatization technology in the analysis of traditional Chinese medicine and food

Analysis of Amino Acids

Analysis of Flavonoids

Analysis of terpenoids

Analysis of antibiotic residues

Analysis of pesticide residues

Analysis of Biotoxins

Analysis of Inorganic Ions

In recent years, the development of chromatography-mass spectrometry technology has shown new prospects for in vivo drug analysis and trace drug analysis. However, chromatography-mass spectrometry technology has high requirements for instrument equipment, strict analysis conditions, and high analysis costs. Currently, it is only applied in all research institutes and difficult to popularize in conventional testing institutions. However, the determination of residual pesticides and biotoxins in traditional Chinese medicine, food, drinking water, and the environment, as well as the determination of residual antibiotics in feed and meat animal tissues, are issues that inspection agencies at all levels must face. Therefore, compared to chromatography-mass spectrometry technology,Post column derivatization technologyAs an important supplement to conventional liquid chromatography analysis, its development and application have significant practical significance in solving the above-mentioned problems.