In biological experiments such as cell culture, protein purification, and liquid chromatography detection, gases such as dissolved oxygen and carbon dioxide in the experimental system are "invisible interference sources" that cause fluctuations in experimental data and a decrease in sample activity.Vacuum DegasserBy using precise negative pressure degassing technology to eliminate gas interference from the root, it has become a key equipment for improving the stability of biological experiments, and its core role can be summarized into three core dimensions.

First move: Remove dissolved gases and avoid sample activity loss

The core objects of biological experiments are often living cells, active proteins, and other environmentally sensitive substances. The dissolved oxygen in the system can trigger oxidative stress reactions, disrupt the spatial structure of proteins, and inhibit mitochondrial respiration, leading to a decrease in cell survival rate and poor experimental reproducibility. The vacuum degassing instrument uses a negative pressure environment to reduce the solubility of gas in liquid, which can quickly remove free oxygen and bubbles from the culture medium and buffer solution. For example, in the purification experiment of monoclonal antibody drugs, the buffer solution treated with degassing can reduce the protein oxidation degradation rate by more than 60%, while avoiding the impact of bubbles on the chromatography column packing, ensuring the stability of the target protein recovery rate.

Second move: Eliminate bubble interference to ensure accurate detection data

In precision experiments such as flow cytometry and enzyme-linked immunosorbent assay, tiny bubbles in the liquid can obstruct the light path, interfere with signal acquisition, and cause false positives or numerical drift in data. The vacuum degassing instrument can preprocess the detection reagents and increase the bubble removal rate to over 98%. Taking real-time fluorescence quantitative PCR experiments as an example, the reaction solution without degassing is prone to uneven fluorescence signals due to bubbles. However, after degassing treatment, the coefficient of variation of Ct values in the same batch of samples can be controlled within 0.5%, greatly improving the consistency of experimental data.

Third move: Maintain system uniformity and strengthen experimental reproducibility

Biological experiments require high uniformity of the reaction system, and local enrichment of dissolved gases can cause microscopic differences in pH values and ion concentrations, leading to deviations in experimental results. The vacuum deaerator is accompanied by gentle stirring during the deaeration process, which can make the reagent components mix more evenly and stabilize the physical and chemical properties of the system. In the experiment of inducing differentiation of stem cells, the induction medium after degassing can improve the consistency of cell differentiation direction by 50%, and reduce the fluctuation range of differentiation efficiency in different batches of experiments to ± 3%.

At present, domestic vacuum degassing equipment has achieved technological breakthroughs, with the advantages of miniaturization and intelligence, and can adapt to the degassing needs of multiple samples and scenarios in the laboratory. with the help ofVacuum DegasserThe standardized processing of biological experiments can effectively eliminate gas interference, laying a solid foundation for the reliability and reproducibility of scientific research results.