Evaporative light scattering detectorIt is a universal detector that can detect organic substances without UV absorption. The use of mobile phase low-temperature evaporation technology, new photomultiplier tubes, and semiconductor laser light sources has improved sensitivity; High precision gas flow control and evaporation temperature control reduce signal noise.
Evaporative light scattering detectorThe work process is mainly divided into three stages:
1. Atomization stage:
The mobile phase flowing out of the chromatographic column and the sample mixture are passed through an atomizer to form uniform small droplets under the action of a high-speed airflow (usually nitrogen or air). This process converts liquid samples into aerosols, facilitating subsequent solvent evaporation.
2. Evaporation stage:
Aerosols enter the heated drift tube, and the mobile phase (solvent) evaporates at high temperatures, while the sample components form small solid or liquid particles due to their lower volatility than the mobile phase. These particles enter the detection tank with the airflow.
3. Detection stage:
The laser beam in the detection pool is irradiated onto the particles, causing them to scatter light. Scattered light is received by photomultiplier tubes or other photodetectors and converted into electrical signals. The signal strength is directly proportional to the number and size of particles, reflecting the concentration of sample components.
The evaporative light scattering detector can be applied to detect all samples with lower volatility than the mobile phase, regardless of whether they have UV absorption or fluorescence characteristics. Therefore, it can be used to analyze compounds that are difficult for traditional detectors to detect, such as sugars, lipids, polymers, amino acids, vitamins, etc.