Preface

Matsutake alcohol is an active ingredient in matsutake mushrooms, which has various health benefits. Firstly, matsutake alcohol has antioxidant properties, which can eliminate free radicals and prevent cell oxidation and aging. Secondly, matsutake alcohol can enhance immunity, promote the production and function of immune cells, thereby improving the body's resistance. In addition, matsutake alcohol can also reduce blood lipids, blood sugar, and prevent cardiovascular and cerebrovascular diseases and diabetes. Finally, matsutake alcohol also has anti-tumor effects, which can inhibit the growth and spread of tumor cells and have a certain auxiliary effect on tumor treatment.

As a characteristic flavor substance of edible mushrooms, the accurate quantification of matsutake alcohol content can effectively promote the quality evaluation, production and reprocessing of edible mushrooms, improve the quality standard system of edible mushrooms, and enhance the market competitiveness of edible mushroom products. At present, the detection of matsutriol is mainly focused on edible fungi and various plants, and its determination is mainly carried out through gas chromatography-mass spectrometry (GC-MS) method. However, most determinations use matsutriol as one of the many components for non targeted scanning and qualitative analysis, without precise quantification. Therefore, there is an urgent need to develop a fast and convenient method for extracting and accurately detecting matsutake alcohol.

fromLed by Kunming Edible Fungi Research Institute of All China Federation of Supply and Marketing Cooperatives，Collaborated with multiple professional organizations such as Thermo Fisher ScientificJointly formulatedIndustry standard GH/T 1486-2025 "Determination of Matsutake Alcohol Content in Edible Fungi by Gas Chromatography"Will be2026年1月1日起Across the countryofficially implementedThis standard meets the technical level of testing practitioners and can be used by major chemical analysis laboratories in China to meet the specified requirements. The method has universal applicability and is easy to promote and use.

As one of the drafting units of the standard, Thermo Fisher participated in the standard preparation and conducted extensive experimental verification using Thermo Fisher Trace1600 series gas chromatography combined with hydrogen flame ionization detector. The verification parameters included calibration curve, spiked recovery rate, precision, sample determination results, and other parameters.

Results and Discussion

Chromatogram and standard curve

This method uses the external standard method for quantification. After analyzing the prepared standard solution on the machine, a standard curve is plotted with the total concentration of the standard solution as the horizontal axis and the total peak area of the standard solution as the vertical axis. The typical chromatogram of matsutake alcohol is shown in Figure 1, and the spectrum of edible mushroom samples is shown in Figure 2.

Figure 1 Chromatogram of Matsutake Alcohol Standard Solution (Click to View Large Image)

Figure 2: Spectrum of Fresh Mushroom Samples (Click to View Large Image)

In this method, two chromatographic columns, TG-WAXMS and TG-17SILMS, were compared. Both columns showed good testing performance for matsutake alcohol, but TG-17SILMS column had better retention effect and higher instrument response value. Therefore, TG-17SILMS column was selected for this method. From Figure 2, it can be seen that during the actual sample testing process, matsutake alcohol was not affected by the matrix and achieved good separation from the matrix peaks in the sample.

Matsutake alcohol (1-octen-3-ol) standard working solution: Ethanol is used as a diluent to prepare standard working solutions of 0.05 μ g/mL, 1 μ g/mL, 3 μ g/mL, 5 μ g/mL, and 10 µ g/mL at each level, which are prepared on site for immediate use.

Figure 3 Matsutake Alcohol Calibration Curve (Click to View Large Image)

Table 1 Calibration curve and correlation coefficient of matsutake alcohol

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Precision and recovery rate

precision

In order to verify the precision of the method, three types of dried and three types of fresh edible mushroom actual samples were selected, and each sample was processed in parallel and measured 6 times. The RSD of the edible mushroom samples obtained in the test results was 2.52%~5.93%, indicating that the precision of the method is good.

Table 2 Precision Testing of Fresh Edible Fungi

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Table 3 Precision Testing of Dried Edible Fungi

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Recovery rate verification

Accurately weigh shiitake mushrooms (fresh), truffles (fresh), and matsutake mushrooms (dry) separately, select 3 spiked levels, and measure 4 parallel levels for each spiked level. Extract and continuously measure according to the pre-processing steps, and calculate the recovery rate. The results showed that the three-level spiked recovery rates of each sample matrix were between 82.0% and 107%.

Table 4 Recovery rate verification test

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Conclusion

This method uses the new generation TRACE 1600 series GC from Thermo Fisher Scientific for experiments, which can fully meet the requirements of detection sensitivity and precision. At the same time, this method has the advantages of simple and fast pretreatment, high recovery rate of spiked samples, high instrument detection sensitivity, and good linear range, which can meet the detection needs of matsutriol in edible mushrooms.

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