1Gene chip membrane analyzerDaily cleaning: prevent minor issues and prolong the lifespan of components

Shell and operating interface

After daily use, use an anti-static soft cloth dipped in neutral cleaning agent (such as 75% medical alcohol diluent) to gently wipe the outer shell, avoiding the use of organic solvents such as acetone and ethanol to corrode the surface coating.

The operation panel (such as a 7-inch touch screen) needs to be wiped with a dedicated cleaning stick or lens paper to prevent scratches.

Deep cleaning of pipetting system

Daily maintenance: After the experiment, rinse the transfer channel with deionized water for 3-5 minutes to remove residual reagents (such as nucleic acids and proteins) and prevent crystallization from blocking the nozzle.

Weekly maintenance: Soak the pipette module in a 0.05% BioTerge solution for 20 minutes to dissolve organic deposits, especially for high salt or corrosive samples.

Key components:

Nozzle: Soak in JetWash solution and blow dry with compressed air to avoid oxidation caused by liquid residue.

Sealing ring: Check whether the O-ring is aging or deformed, replace it once a month to prevent leakage.

Optical component maintenance (if equipped with detection module)

Dip a dust-free cloth in isopropanol and gently wipe the lens every week to avoid fingerprints or dust affecting the imaging quality.

When not in use for a long time, the lens should be stored in a dry dish with humidity controlled at 30% -50%.

2、 Regular calibration: ensuring accurate and reliable data

Pipette accuracy calibration

Once a month: Use a standard solution (such as 0.1M NaCl) for three-point calibration (50 μ L, 100 μ L, 200 μ L), with an error margin of ≤± 2%.

Calibration tool: Axygen suction head (compatibility verified by the manufacturer) and dedicated calibration board are used to avoid system errors caused by third-party consumables.

Mechanical arm positioning calibration

Once every quarter: Run the self check program to check the X/Y/Z axis motion accuracy, and the positioning deviation should be ≤ ± 5 μ m.

Adjustment method: Fine tune parameters through software (such as stepper motor pulse count), or contact the manufacturer for hardware calibration.

Environmental parameter monitoring

Record the laboratory temperature and humidity daily (recommended 15-30 ℃, humidity 30-65% RH), and activate the temperature and humidity control system when exceeded.

Equipped with UPS power supply to prevent voltage fluctuations (within ± 10%) from damaging electronic components.

3、 Consumables Management: Standardized Operations to Reduce Losses

Suction head and microplate

Only use compatible models (such as Axygen 1000 μ L filter cartridge suction head) to avoid nozzle clogging or pipetting deviation caused by non-standard consumables.

When loading the suction head, gently press it into place to avoid damaging the spring structure with excessive force.

Reagent and solution management

Before the experiment, check the validity period of the reagents. Expired or turbid solutions should be discarded.

Highly volatile reagents (such as methanol) need to be sealed and stored to reduce the risk of crystallization in pipelines.

waste liquid treatment

Clean the waste liquid tank daily to avoid long-term storage of corrosive liquids (such as strong acids and alkalis) that may damage the container.

Rinse the waste liquid pipeline with deionized water every month to prevent blockage.

4、 Preventive maintenance: Establish equipment health records

Three level maintenance system

Daily inspection: power on self-test, nozzle cleaning, waste liquid cleaning.

Weekly maintenance: flushing of transfer channels, inspection of sealing rings, and backup of operation logs.

Monthly maintenance: precision calibration, cooling fan dust removal, power cord contact inspection.

Fault warning mechanism

Record abnormal phenomena (such as increased noise and slower pipetting speed) and analyze potential issues through device logs.

For example, if the calibration error exceeds the standard for three consecutive times, it is necessary to check for nozzle wear or motor drive board failure.

Spare parts inventory management

Reserve commonly used vulnerable parts (such as nozzles, seals, and filters) to ensure that they can resume operation within 24 hours after a malfunction.

Spare parts should be stored according to the manufacturer's requirements (such as avoiding light and moisture) to avoid premature aging.

5、 Operating standards: Reduce human errors

Standardized process

Strictly follow SOP and prohibit skipping self inspection or calibration steps.

Example: Before sampling, it is necessary to confirm the flatness of the substrate to avoid nozzle collision caused by substrate warping.

personnel training

New operators need to undergo manufacturer certification training to master equipment principles and emergency handling (such as quick cleaning of nozzle blockages).

Regularly organize refresher training to update and maintain knowledge (such as the use of new consumables).

safety protection

　　Gene chip membrane analyzerWear gloves during operation to avoid skin oil contamination of optical components.

After the experiment, turn off the device power and unplug it to prevent lightning damage to the circuit.