Determination of Vitamin A, D, and E in Food (Second Method)

The GB 5009.82-2016 "Determination of Vitamin A, D, and E in Food" issued by the National Health and Family Planning Commission and the State Food and Drug Administration of the People's Republic of China on December 23, 2016 was officially implemented on June 23, 2017.
The new standard * method is applicable for the determination of vitamin A and vitamin E in food; The second method is applicable for the determination of vitamin E in foods such as edible oil, nuts, beans, and chili powder; The third method is applicable to the determination of vitamin D2 and vitamin D3 in food; The fourth method is applicable to the determination of vitamin D2 or vitamin D3 in formula foods.
So we know that before analyzing and testing food samples, pre-treatment of the samples is necessary, and the pre-treatment time of the samples may occupy 60% of the entire testing process, and 50% of the final result error may come from the pre-treatment process. So, how can IKA's pre-processing equipment help experimenters improve the efficiency and accuracy of pre-processing in response to the new standards? Let's taste IKA's pre-processing solutions through the interpretation of the new standards!

Method 2: Determination of Vitamin E in Food by Positive Phase Liquid Chromatography

Experimental Procedure

Sample Preparation ->Sample Processing ->Sample Measurement
GB 5009.82-2016 Requirements for Sample Preparation:
1.1 Sample preparation: After the sample is divided, crushed, or homogenized, it is stored in the dark and refrigerated

A11 grinder

Suitable for grinding different types of samples
Easy to operate

T25 digital disperser

Suitable for homogenization of different types of samples such as lipids and legumes
Special serrated blade to solve the problem of sample winding

1.2 Sample processing: Plant oil: 0.5-2g oil sample in 250ml volumetric flask -->Add 0.1g BHT -->10ml mobile phase vortex oscillation dissolution -->Filter and wait for injection

Vortex 2 vortex mixer

Jogging and continuous operation mode for two weeks
Optional fixtures suitable for volumetric flasks
500-2500 rpm speed continuously adjustable

Cream and butter: 2-5g of sample in a 50ml centrifuge tube ->add 0.1g BHT, melt in a 45 ℃ water bath ->add 5g anhydrous sodium sulfate, vortex for 1 minute ->add 25ml mobile phase vortex oscillation extraction ->centrifuge ->transfer the supernatant to a concentration bottle, distill under reduced pressure on a rotary evaporator in a 45 ℃ water bath to 2ml, dry the mobile phase dissolution residue with nitrogen, and use it for liquid chromatography determination

ICC basic eco18 constant temperature water bath

RT~100 ℃, meets the melting requirements of the sample
Transparent bath, easy to observe the melting of the sample
Optional floating plate for fixing 50ml centrifuge tube

MS 3 digital vortex mixer

Timer function to ensure repeatability
Jogging and continuous operation modes
Up to 3000rpm for better dissolution and extraction efficiency

RV10 auto rotary evaporator

Automatic boiling point recognition function, realizing distillation of unknown solvents and improving yield

Dried plant samples such as nuts, beans, and chili powder: Weigh 2-5g of the sample ->Use a Soxhlet extractor to extract plant oils ->Transfer the extraction solution to a 250ml evaporation flask and distill under reduced pressure in a 40 ℃ water bath to dryness ->Transfer 10ml of the mobile phase oil to a 25ml volumetric flask ->Add 0.1g BHT vortex shaking dissolution ->Filter and wait for injection.

Soxhlet extraction kit (RET heating magnetic stirrer+heating sleeve)

The IKA Soxhlet extraction solution saves you high costs. All you have purchased is a universal magnetic stirrer and a heating sleeve
Long term safe operation

Program control ensures the repeatability of batch samples