Application of Microscopic Counting Insoluble Particle Analyzer in Liposome Detection

liposome(Liposomes)It is a liposome made from phospholipids and ceramides(hollow)It has a bilayer structure similar to the skin cell membrane structure and has excellent moisturizing effects on the skin, especially liposomes coated with moisturizing substances such as hyaluronic acid and polyglucoside, which are more excellent moisturizing substances.

Liposomes are classified into single chambered liposomes and multi chambered liposomes based on the number of layers of lipid bilayers they contain. Small single compartment liposome(SUV): Particle size approximately0.02～0.08μm; Single compartment liposomes(LUV）Single layer large vesicles with particle size0.1～1 μm.

Therefore, quality control of liposomes is an important indicator, and the particle size characterization of liposomes mainly includes the characterization of average particle size and insoluble particles. This article will focus on introducing the inspection methods for insoluble particles.

Introduction to the regulations for the examination of insoluble particles in liposomes:

Insoluble particle inspection, mainly referring to the Chinese Pharmacopoeia2020Version andUSP788Chapter. These two regulations introduce two methods for detecting insoluble particles:Photoresist method andMicroscopic counting method.

For the examination of liposomes, the current Chinese Pharmacopoeia mainly refers to the Chinese Pharmacopoeia2020edition0903The chapter and regulations do not explicitly mention which method liposomes should refer to, butUSP 788It is explicitly mentioned that samples such as liposomes, emulsions, and colloids need to be examined using microscopic counting methods.

Compared to microscopic counting methodThe photoresist methodThere are special advantages in liposome examination, the first of which isIt is necessary to consider the influence of sample concentration and reflect the true insoluble particle results of the system; Second noReceived samplesThe influence of packaging quantity, microscopicCounting method for the whole unitCheck; The third microscopic counting method can not only see the size of particles, but alsoObserving the morphology of particles is of great help for tracing and improving the process of insoluble particles.

Application case of liposome insoluble particle examination:

For the above-mentioned liposome samplesCheck the concentration limit, lightIf the obstruction methodDirect testing can result in many false results, as high particle concentration can cause particle overlap and adhesion, leading to unsatisfactory test resultsnostable.The microscopic counting method has no relevant concerns for the examination of liposomes, and directly filters the examination to reflect the true insoluble particle results of the sample.The figure below shows胤煌technologyYH-MIP-0103Manufacturing of semi-automatic equipmentSample processAnd the test results. newly developedYH-MIP-0205ProThe product has eliminated the manual sample preparation step and now integrates automatic sample loading, automatic filtration, and automatic dryingIntegrating automatic testing to further improve inspection efficiency.

Introduction to Testing Instruments:YH-MIPMicroscopic Counting Insoluble Particle Analyzer

YH-MIP-0103The microscopic counting image method particle size analyzer has solved the problems of insufficient accuracy, cumbersome operation, significant harm to human eyes, and untraceable detection results in traditional microscopic testing methods. It is a new generationYH-MIP-0205 ProtypeMicroscopic counting image method particle size analyzer has the technological advantages of the previous generation of instruments，Newly optimized, next-generation productCapable of detecting insoluble particles in samples in a fully automated manner！

胤煌technology(YinHuangTechnology)We are a high-tech company dedicated to providing testing and analysis equipment and technical services for industries such as pharmaceuticals, semiconductors, and chemical materials. We are committed to providing customers with comprehensive and accurate testing, analysis, and solutions. Our main products include insoluble particle analyzer, visible foreign matter inspection analyzer, raw liquid particle size andZetaPotential analyzer,CHDFHigh precision nanometer particle size analyzer, high-resolution nanometer particle size analyzer, solution color analyzer, clarity analyzer, etc., independently developed by the companyYH-MIPSeries Microscopic Counting Insoluble Particle AnalyzerYH-FIPSSeries flow dynamic image method particle size analyzer,YH-FIPSSeries microfluidic imagingParticle analyzers have been widely used in the fields of biomedicine, semiconductors, and materials chemistry.

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