As a core consumable for microbial culture and cell expansion, the triangular oscillation culture bottle (triangular oscillation bottle) needs to be deeply integrated from three dimensions: standardized interface design, compatibility with closed transfer systems, and modular functional expansion to adapt to automated liquid processing workstations and high-throughput screening systems.
1、 Standardized interface and size adaptation
Triangle shaking bottles need to be standardized to match the fixtures and oscillation modules of automation equipment. For example, the 2L/3L/5L triangular shaking bottle series launched by Jiete Biotechnology meets the ISO8318 international standard for bottle mouth diameter, bottle body height, and bottom curvature, and can seamlessly adapt to the robotic arm grasping system of automated workstations such as Hamilton and EosBot. At the same time, the bottle body is made of high-strength polypropylene (PP) material to ensure that it does not deform under high-speed oscillation (such as 250rpm), avoiding liquid splashing or liquid transfer errors caused by container shaking.
2、 Integration of Closed Transfer System
To reduce the risk of cross contamination, the triangular shaking bottle needs to support sterile and sealed transfer. The closed transfer system of Jiete Biotechnology is designed with dedicated bottle caps and silicone sealing rings, which can be directly connected to the pipette or pipeline of the automated workstation, achieving sterile transfer of liquids between shaking bottles, microplates, and fermentation tanks. For example, in the vitamin C mixed bacterial fermentation experiment, the system can complete 200 liquid transfers within 48 hours with a colony contamination rate of less than 0.1%, significantly better than traditional open pipetting methods.
3、 Modular function expansion
The triangular shaking bottle needs to reserve functional interfaces to support the diverse needs of high-throughput screening. For example, some models of shake flasks can be equipped with pH/DO (dissolved oxygen) sensor modules to monitor the cultivation environment parameters in real time, and transmit the data to the control software of the automation workstation (such as EosBot) through Bluetooth or wired connection ® LH96's temperature controlled oscillation module enables dynamic adjustment of cultivation conditions. In addition, a magnetic stirrer can be integrated at the bottom of the shaking bottle to adapt to the magnetic purification module in high-throughput screening systems, simplifying protein extraction or nucleic acid separation processes.