Research progress on filtration membrane technology in the field of microbial drug separation and purification
Date: 2016-02-18Read: 26
（1） The improvement of filtration membrane is the core of separation technology, and the differences in the morphology and structure of filtration membrane are closely related to its mechanism, which directly affects its application in extraction process. The performance of separation filtration membranes includes selectivity, permeability, physical and chemical stability. The selectivity depends on the difference in the rate at which the separated substance passes through the filter membrane, representing the separation efficiency of the filter membrane. Transmittance refers to the magnitude of the filtration rate (also known as flux) of a filter membrane, which is an indicator of the membrane's processing capacity. It is difficult to meet both of the above requirements for a single polymer filter membrane. So chemical or physical methods are often used to improve the performance of filter membrane materials, so that the filter membrane has certain required properties to improve separation efficiency. Radiation grafting is one of the ways to modify the surface of filtration membranes. Shim. J K et al. used gamma ray radiation to graft hydrophilic methyl methacrylate 2-hydroxyethyl ester monomer onto the surface of a polypropylene ultrafiltration membrane. The modified polypropylene membrane was then used for ultrafiltration treatment of bovine serum albumin solution, and it was found that the solution flux increased, the fouling resistance of the filtration membrane was enhanced, and the hydrophilicity of the filtration membrane surface was also enhanced.
（2） The development and optimization of filter membrane components require not only specific properties of filter membrane materials for a specific separation process, but also consideration of changes in field quantities along the components. The development and optimization of filtration membrane components involve finding the appropriate filter membrane carrier and suitable operating conditions, among other factors. When optimizing filter membrane components, in addition to measuring the characteristics of the filter membrane through experiments, it is usually necessary to calculate tedious data, and sometimes it is also necessary to study and explore the permeation mechanism. Kawasaki et al. conducted a series of preliminary experiments to explore the possibility of extracting erythromycin using liquid filtration membranes. They compared the extraction efficiency of many solvents for erythromycin and considered factors such as water solubility and toxicity. Eventually, n-decanol was selected as the carrier for the filtration membrane, with a partition coefficient of 122 for erythromycin aqueous solution. At the same time, the permeation mechanism of erythromycin was also studied. Experimental data showed that when the pH of the solution was less than 6, erythromycin existed almost in proton form, and when the pH was greater than 10, it became a neutral molecular form. So the experiment used a porous 48%, 0.02 micron CORETEX (mesh plastic thin filtration membrane) as the support, and n-decanol as the carrier to extract the liquid through the filtration membrane from the alkaline fermentation broth. Then, an acidic buffer containing 0.025mol citric acid, 0.10mol boric acid, and 0.05mol sodium phosphate per liter was used to remove the proton form. As a result, erythromycin can pass through the liquid filtration membrane and reach the acidic buffer solution.
（3） To successfully complete a separation task, the design of a filtration membrane device often requires the joint use of several filtration membrane components. If high-quality products are required, multi-stage devices must be designed, which is the design of a filtration membrane device. A good filtration membrane device not only needs to consider the arrangement of series and parallel connection between filtration membrane components, but also needs to consider the ability to better cooperate with other separation methods in order to achieve the requirements of the highest final product quality. In recent years, foreign research and development have used 0.2um GVLP (filter membrane brand produced by MILLAPORE) microporous filtration membrane to filter cephalosporin C fermentation broth (MF), removing mycelium and solid matter from the culture medium. The obtained filtrate is then subjected to another set of PTGC filtration membrane ultrafiltration (UF) system to remove some proteins, polysaccharides, and some dark brown pigments with molecular weights above 10000 from the filtrate, in order to obtain a lighter and purer head C filtrate, which is then concentrated using a polyamide reverse osmosis filtration membrane (RO). Through further purification by HPLC, a relatively pure cephalosporin C can be isolated. If combined with enzymatic lysis of cephalosporin C, 7-ACA can be directly produced without isolating the cephalosporin C product, making the separation, purification, and lysis of cephalosporin C a one-stop process and greatly simplifying the production process of semi synthetic cephalosporins.
Aseptic single piece filter membraneparameter
Trademark Name: S-Pak
Quantity/Packaging: 600
Application: Microbial Analysis
What is the pore size of the filter membrane,? m:0.45
Packaging: S-Pak filter membrane, individually sealed, with blue filter membrane paper, sterile substitute: HAWG 047 PP filter membrane diameter, mm:47
Filter membrane material: mixed cellulose ester
Filter membrane color: white
Filter membrane surface: grid
Aseptic: Aseptic