How to choose a Flash column?

About Us

Shanghai Tongwei Analysis Technology Co., Ltd

E-mail
info@unimicrotech.com.cn
Phone
Address
Building C01, No. 489 Songtao Road, Zhangjiang High tech Park, Pudong, Shanghai

Date: 2023-02-09Read: 0

FlashColumn selection

Example: Alkaline compoundsFlashColumn selection

This compound contains phenolic hydroxyl groups, secondary amines, and tertiary amine structures. It is a strongly polar alkaline compound that interacts significantly with the silicon hydroxyl groups on the surface of silica gel, resulting in tailing on TLC silica gel plates. Therefore, ordinary silica gel columns are not suitable for the separation and purification of this type of compound. In contrast, amino columns are more suitable for the separation and purification of this type of alkaline compound.

Example: Acidic compoundsFlashColumn selection

This compound contains phenolic hydroxyl, amino, and carboxyl structures, and is a strongly polar acidic compound. It can also interact with the silicon hydroxyl groups on the surface of silica gel due to acid dissociation on ordinary silica gel columns, resulting in tailing. Therefore, diol based columns are more suitable for the separation and purification of this type of compound.

YamazenIt is a Japanese brand with over 40 years of experience in low-pressure preparative chromatography, and has many loyal users such as GlaxoSmith Kline, Novartis, Pfizer, Roche, AstraZeneca, etc. The products are sold to various related research institutions and enterprises in North America, the United Kingdom, South Korea, Australia, Southeast Asia and other countries, occupying an important position in the field of low-pressure preparative chromatography.

Last Article: Why does the laboratory need to use an air generator?

Next Article: Determination of 17 amino acids by HPLC-UV pre column derivatization method