Weigh glucosamine hydrochloride standard1.0 gDissolve in water and bring to volume100 mLIn a volumetric flask, prepare to a mass concentration of10 mg/mLReserve solution. Measure an appropriate amount of reserve solution separately and dilute to volume10 mLIn a volumetric flask, a series of mass concentrations were obtained1.0 mg/mLThe1.5 mg/mLThe2.0 mg/mLThe2.5 mg/mLThe3.0 mg/mLThe3.5 mg/mLGlucosamine hydrochloride standard solution.