Real time quantitative PCR instrumentLabeling DNA products with fluorescent chemicals (such as fluorescent dyes or probes) to capture real-time changes in fluorescence signal intensity during PCR index amplification. The system automatically collects fluorescence increment data for each cycle, and after baseline correction and threshold setting through supporting software, calculates the initial template concentration using standard curve or Δ Δ Ct method. This technology has achieved a leap from qualitative to quantitative PCR, and the entire PCR process can be automated, with short time consumption, easy operation, and less pollution.

　　Real time quantitative PCR instrumentSelection suggestion:

When choosing a real-time quantitative PCR instrument, the following factors should be considered comprehensively:

Experimental requirements: Clarify the type of target molecule to be detected, detection sensitivity requirements, and platform applicability. For example, gene expression analysis can choose a 4-channel system, while virus load detection is recommended to choose the ROX correction dye channel.

Performance indicators: Focus on the accuracy, sensitivity, amplification speed, and uniformity of the temperature control system. The better the performance of the PCR instrument, the higher the experimental stability and accuracy.

Brand and after-sales service: Choose brand products to ensure product quality and reliable after-sales service. At the same time, consider the application scope and accessory selection of brand products.

Budget: Choose suitable instruments based on the budget. In the case of limited budget, a PCR instrument with stable performance and high cost-effectiveness can be chosen.