Many hospitals and related departments in China often overlook the role of washing machines. In fact, as long as you truly understand this instrument and equipment, you can know its function and bring you more benefits. Washing machine is a widely used medical device. It is very important for hospitals and some experimental departments to use washing machines to wash the enzyme-linked immunosorbent assay (ELISA) analyzer and culture plates during the coal standard detection process.
The washing machine has a certain number of washes, and if multiple washes are beneficial for achieving better results, 6 washes are recommended. When washing, pay attention to the amount of liquid added to each well. It is advisable to fill it up, but not overflow. Generally, it is controlled at 300 microliters per well. It is recommended to adjust the position before washing, and the suction needle should be at the bottom of the micropore to reduce the residual amount of washing solution. Because the general ELISA experiment requires the plate to be clipped after washing, but this step can easily cause contamination in PCR-ELISA, so the residual amount should be minimized as much as possible. Do not use force to clamp the plate like in general ELISA operations. Users should use two-point liquid suction and bottom flushing to achieve better washing results. If using a PCR-ELISA liquid tank, 1% sodium hypochlorite should be added, and the amount added should not be less than the amount of waste liquid generated. After daily use, rinse the pipeline and wash the hair with 1% sodium hypochlorite, and then rinse the pipeline with double distilled water.
Maintenance and upkeep of the washing machine:
1. When the washing machine is idle, the power plug should be unplugged.
2. At the end of using the washing machine, the inside and outside of the washing machine should be cleaned. Clean all pipelines with distilled water to prevent crystallization of the washing solution from blocking the pipelines and affecting normal use.
3. After the work is completed, the waste liquid should be promptly disposed of to prevent the waste liquid bottle from being too full and causing the waste liquid to reflux, resulting in the failure of the washing machine.
4. Ensure that the edges of the holes on the reaction plate to be washed are not too high and level during work, in order to prevent damage to the suction needle. The washing solution and waste liquid pipelines should be dismantled and flushed regularly to ensure the smoothness of the pipelines.