High pressure sterilizerIt is a high-pressure container used to sterilize liquids or instruments by raising the boiling point of water to a temperature above 100 ℃ at a pressure higher than normal pressure. High pressure sterilizers belong to mandatory testing instruments, but mandatory testing only assesses the physical parameters of the instrument. So sterilizers that have undergone strong testing must also undergo sterilization effectiveness verification. But most units often overlook this issue. Today, let's talk about how to verify the effectiveness of high-pressure sterilizers.
Verification method:
Place the thermophilic Bacillus subtilis disk (hereinafter referred to as the bacterial disk) into a sealed test tube using sterile forceps. Put the chemical indicator card and the retention thermometer into an open test tube. Prepare 5-10 portions of each of the above two types of test tubes. Place them separately at the steam outlet, bottom exhaust outlet, and bottom water outlet of the high-pressure sterilizer, or at the top, bottom, left, right, and middle 5 positions. If the sterilizer is on the second floor, it needs to be placed in 10 places.
The temperature gauge can only be used for verification testing after being standardized and qualified. Before testing, the mercury column of the thermometer needs to be shaken to below 40 ℃. After each monitoring, if the temperature difference of the thermometer should be between 1 ℃, it indicates that the temperature distribution inside the sterilizer is uniform.
The sterilized bacterial slices should be placed in sterilized bromocresol purple peptone water culture medium at 56-60 ℃ for 24-48 hours under strict aseptic operating conditions, and the color change should be observed. If the culture medium turns yellow, it indicates that the thermophilic fat loving Bacillus subtilis in the bacterial slide has not been inactivated, and the bacteria can still grow in the culture medium, decomposing glucose to produce acid and turning yellow. If the color of the culture medium remains purple without changing, it indicates that the spores have been inactivated. At the same time, unsterilized paper should be placed in the culture medium as a positive control, and blank culture medium without paper should be used as a negative control.
The indicator color block on the chemical indicator card changes from light yellow to black during high-pressure steam sterilization. As the color changes in depth, and compared with the control color, it can be determined whether the sterilization effect meets the requirements. Chemical indicator cards should be stored in a dry place. It will change color when exposed to moisture, affecting the observation of sterilization effect.
High pressure steam sterilization requires the steam to smoothly enter the sterilizer, come into contact with the sterilized items, and exhaust the existing cold air in order to achieve the sterilization effect. To verify the no-load thermal distribution and full load thermal penetration (not exceeding 2/3 of the total volume at full load). Two types of verification are repeated three times each, for a total of six times.
Six experiments at five points showed that the temperature was all at 121 ℃, and the chemical indicator card turned black; The degree is consistent with the control color; The color of the culture medium did not change, indicating that the high-pressure steam sterilization effect is qualified.
Apart from thisHigh pressure sterilizerWhen working, it is required to use distilled water or pure water because distilled water has the characteristics of purity, no impurities, no ions, and no scale formation. If distilled water is not used, it will cause damage to the machine If the fine pipeline is blocked, scale and water spots will form on the surface after drying, and deposit on the surface of the steam sensor and temperature sensor inside the sterilizer, causing the sensor to malfunction and affecting the normal use of the sterilizer.