Analysis of the method for manually tuning the half width of 4500, 5500, and 6500 series mass spectra using OS software
Date: 2025-12-03Read: 0
This article takes 6500+Q1 positive ions as an example to introduce the method of manually tuning the full width at half maximum of 6500+mass spectrometry using OS software. This method is also applicable to 4500 and 5500 series triple quadrupole mass spectrometry operated using OS software.
The Power of Precision. ///
Enter the MS Tuning interface and select MS Scan Tuning from the drop-down menu in the Tuning Procedures column
The Power of Precision. ///
Enter the MS Tuning interface, select Tuning and Calibration MS, and then choose the items that need to be tuned. Taking the Q1 positive ion 10Da/S scan rate as an example, the following choices are made:
Polarity: Positive ;
Scan type: Q1;
Resolution: Unit;
Scan rate: 10
The Power of Precision. ///
After the needle pump is filled with positive ion tuning liquid, click the needle pump control button to open the control window, set the flow rate to 5ul/min, click Start to run the needle pump, and then click Next at the lower right corner to enter the next step to obtain stable spray (Achieve Stable Spray)
Note:
1) Positive ions of 5500+and 6500+use Pos PPG 2E-7M, while negative ions use PPG 3E-5M;
4500 positive ions using Pos PPG 2E-6M, negative ions using PPG 3E-4M
2) The positive ion flow rate is 5ul/min, and the negative ion flow rate is 10ul/min
The Power of Precision. ///
After observing the stable total ion current on the left and the 906 mass spectrum on the right, click Stop first, and then click Next in the bottom right corner to proceed to the next step of optimizing the ion sourceGas1（ Optimize Source Gas1）
The Power of Precision. ///
The software automatically optimizes Gas1, and after optimization is completed, it automatically enters the initial Q1 calibration at 10 Da/sec
The Power of Precision. ///
The software automatically completes the initial Q1 calibration of 10 Da/sec and then enters the tuning Q1 positive 10 Da/sec mode
The Power of Precision. ///
After entering the tuning Q1 positive 10Da/sec step, the software automatically optimizes the half peak width of each ion. If manual optimization is required, click the Auto Calibrate stop button, light up the calibration button to enter manual calibration mode, optimize the offset and adjust the half peak width. It is recommended to take a photo and record the initial offset value before optimization
The Power of Precision. ///
Adjust the offset value according to the up or down arrow to optimize the full width at half maximum of each ion to achieve the required index of 0.64-0.74. The initial half width of ion 500.380 in the following figure is 0.85, which is greater than the maximum value of the indicator 0.74. By continuously pressing the upward arrow 3-5 times and observing the new half width of the collected ion in the mass spectrum, it can be determined whether to continue the above operation until the actual half width of ion 500.380 falls between 0.64-0.74.
Note:
1) If the actual half width of the ion is less than 0.64, the half width can be increased by pressing the downward arrow;
2) The number of times to adjust the button can be adjusted appropriately according to the size of the offset.
The Power of Precision. ///
After adjusting the full width at half maximum of each ion to 0.64-0.76, press the Comfirm button in the upper right corner, and the software will enter the step of confirming Q1 positive 10 Da/sec (Comfirm Q1 Positive 10 Da/sec) to confirm and complete the tuning
The Power of Precision. ///
The Power of Precision. ///
Click Save Report as to save the Q1 positive ion 10Da/S tuning report, and then close the tuning interface
