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Phosphate buffer containing Tween 20 (PBST)
Date: 2025-07-15Read: 4

PBST (Phosphate Buffered Saline with Tween 20) Phosphate buffer solution containing Tween-20 can provide a relatively stable ion environment and pH buffering ability, and is a commonly used buffer salt solution in biology. It is mainly used in immunohistochemistry, in situ hybridization, enzyme-linked immunosorbent assay and other experiments to clean immunoblotting membranes. PBST contains non-ionic detergent Tween 20, which inhibits protein and immune analysis programs such as enzyme-linked immunosorbent assay (ELISA) and Western blotting to reduce non-specific binding and staining during the cleaning step, resulting in clearer results and spots.

Phosphate buffer containing Tween 20 (PBST) can provide two forms of products: safe and convenient tablets and truly uniformly mixed instant granules.