Chicken Luteinizing Hormone LH Kit

The quotation, consultation and selection, technical service, and selection of the correct experimental reagents or materials for the chicken luteinizing hormone LH test kit are the key to the success of the experiment.

1、 The customer does not open the lid when receiving the cells, and leaves them in the incubator for several hours (depending on the cell density) to observe the growth of the cells under an inverted microscope, and takes photos of the cells at different magnifications (it is recommended to take a photo of the culture medium when receiving the cells, observe the color of the culture medium and whether there is any leakage, and take one photo of the cells at 100X and 200X under the microscope), to eliminate contamination of the cells themselves;

We are responsible for sending a free cell line in case of contamination due to unopened cells received.

2、 If it is an adherent cell, please observe the cell density under a microscope:

1. When the confluence degree does not exceed 80%, use 75% alcohol (it is recommended to prepare water with 75% alcohol, which is also sterilized ultrapure water), spray the entire bottle for disinfection, and place it in the sterilization table for strict aseptic operation; then open the lid, first suck out about 10ml of the culture medium, place it in the centrifuge tube, and then burn the bottle mouth (it is strictly prohibited to pour it directly) to ensure no contamination. Then use a 10ml pipette to suck out the remaining culture solution and place it in the centrifuge tube. Only about 10ml of culture solution is left in the culture bottle. After 16 hours of cultivation (time adjusted according to cell growth), passage or cryopreservation.

When the confluence exceeds 80%, please subculture according to the cell culture conditions. The specific steps are as follows:

1) Discard the culture medium and wash 1-2 times with 3ml PBS (without calcium or magnesium ions);

2) Add 1ml of digestion solution (0.25% Trypsin 0.53mM EDTA) to a T25 culture bottle, invert and place in a 37 ° C incubator for 1-3 minutes to preheat. Then invert the culture bottle for about 30 seconds and observe the cell digestion under an inverted microscope. If most of the cells become round and dispersed, tap the culture bottle lightly and the cells will immediately fall off;

3) Add 6-8ml of culture medium, aspirate, and transfer to new culture bottles as required.

3、 If it is a suspended cell, aspirate the culture medium, centrifuge at 1000 rpm for 5 minutes, aspirate the supernatant, suspend the cells in fresh culture medium at the bottom of the tube, and transfer them back to the culture bottle.

Attention: When changing the medium, use half of our culture medium and half of yours to avoid poor growth caused by cell adaptation.

4、 What are the situations where cells have problems and can be resent? What are the criteria for judgment?

(1) Various problems encountered during cell transportation, such as cell loss, damage, leakage, etc., and resending;

(2) After the recovery of frozen cells, the vast majority of cells did not survive and reappeared;

(3) After 24 hours of standing, the vast majority of surviving cells did not survive and reappeared;

(4) After thawing frozen cells or allowing surviving cells to stand for 4 hours without opening, if contamination occurs, resend;

(5) It depends on the specific situation.

Three principles of cell culture technology: 1. Use your own set of reagents and do not mix them with others; 2. Observe cells diligently, love and care for them like keeping a pet; 3. Regularly change the liquid and passage, do not "fish for three days, sun screen for two days". The freezing time for cells is 5-7 working days, and for live cells it is 7-15 working days.

Various indicators are complete, covering a wide range or classification, as follows:

1、 Common ELISA kits

1. Cytokine detection kit:

Such as interleukins, selectins, colony-stimulating factors, tumor necrosis factor, interferons, transforming growth factors, chemokines, cytokine receptors, adhesion molecules, growth factors, apoptosis factors, etc

2. Liver fibrosis detection ELISA kit:

Such as fibronectin, hyaluronic acid, collagen, matrix metalloproteinase inhibitors, matrix metalloproteinases, laminin, etc

3. Myocardial infarction detection ELISA kit:

Such as troponin, myoglobin, C-reactive protein, etc

4. Endocrine detection ELISA kit:

Such as thyroid, pancreas, sex hormones, progesterone, testosterone, growth hormone, somatostatin, endothelin, cortisol, osteocalcin, prolactin, adrenocorticotropic hormone, follicle stimulating hormone, estradiol, estriol, 5-hydroxytryptamine, 17 hydroxyprogesterone, etc

5. Autoimmune detection ELISA kit:

Like the thyroid gland, salt water can extract nuclear antigen antibodies (ENA) and anti nuclear antibodies, DNA, Anticardiolipin antibodies, rheumatoid factor, circulating immune complexes, anti islet cell antibodies, trypsinogen, Sm, Bullous pemphigoid, protease, short membrane worm method, liver kidney, liver kidney stomach, muscle membrane antibody, keratin antibody, anti nuclear antibody, anti ribosomal protein antibody, anti poly keratin microfilament protein antibody, anti chain O, anti ovarian antibody, anti smooth muscle antibody, anti mitochondrial antibody, anti myocardial antibody, anti histone antibody, granulocyte elastase, skin antibody, adrenal antibody, glomerular basement membrane, glomerular basement membrane antibody, myeloperoxidase, striped muscle antibody, reticulin antibody, parietal cell antibody, gastric protease, systemic lupus erythematosus, bacterial permeability enhancing factor, etc

6. Tumor biomarker detection ELISA kit:

Such as tumor markers, tissue polypeptide antigens, tumor related factors, pancreatic cancer, rectal cancer, cytokeratin fragments, stomach cancer, ferritin, sugar chain antigen, nerve specific dilute alcohol enzyme, epithelial membrane antigen, breast cancer, human anti mouse antibody, prostate, alpha fetoprotein, liver cancer, colorectal cancer, lung cancer, urine and fecal occult blood detection, carcinoembryonic antigen, β -2 microglobulin, etc

7. ELISA kit for eugenics and fertility testing:

Early pregnancy, neonatal TSH, premature rupture of membranes testing, anti endometrial antibodies, anti cardiolipin antibodies, anti zona pellucida antibodies, anti zona pellucida antibodies, anti ovarian antibodies, anti sperm antibodies, cytomegalovirus, Toxoplasma gondii, rubella virus, delivery prediction, mononucleosis, herpes simplex virus, follicle stimulating hormone, luteinizing hormone, fecal occult blood test strips, HCG, etc

8. Infectious disease detection ELISA kit

Such as Helicobacter pylori, encephalitis B, hepatitis B, hepatitis C, hepatitis D, hepatitis E, hepatitis G, chlamydia, venereal diseases, adenovirus, parvovirus B19, pemphigus, varicella zoster virus, mycoplasma genitalium, typhoid, trachoma, mumps, mycoplasma hominis, measles, rotavirus, epidemic hemorrhagic fever, gonococcus, lyme disease, coxsackie, anti ureaplasma, Legionella, tuberculosis, collagen, condyloma acuminatum, hepatitis A, poliomyelitis, acute pancreatitis urinary trypsin, cholera, respiratory syncytial virus, liver fluke, parainfluenza, pneumonia, herpes zoster, infectious Mononucleosis, laminin, * * *, pertussis, diphtheria, Aike virus, EB virus, Group A streptococcus, etc

9. Special protein detection ELISA kit

Such as immunoglobulin, anti chain O-aso, rheumatoid factor RF, C-reactive protein, microalbumin, beta-2 microglobulin human, ferritin, transferrin, etc

2、 Food Safety Inspection ELISA Kit:

Detection kits for hormones, drugs, mycotoxins, allergen residues, genetically modified products, as well as detection products for microorganisms, vitamins, etc. in food.

3、 Agricultural diagnostic kits for plant viruses, bacteria, fungi, plant hormones, and genetically modified crops

4、 Diagnostic kits for animal and plant diseases, such as pigs, cows, sheep, horses, and other livestock and poultry, as well as pet testing kits