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Precautions for storing samples correctly in laboratory refrigerators
Date: 2025-06-18Read: 1
The laboratory refrigerator is an important equipment for storing biological samples, reagents, drugs, etc. Its correct use directly affects the accuracy of experimental results and the safety of samples.
1、 Preparation before using the laboratory refrigerator:
1. Confirm storage requirements
Choose a refrigerator with the appropriate temperature based on the sample type (such as cells, proteins, DNA, reagents, etc.).
Avoid mixing samples with different storage requirements (normal temperature reagents should not be placed in low-temperature refrigerators).
2. Check the status of the refrigerator
Temperature display: Confirm that the actual temperature is consistent with the set temperature and verify using a calibrated thermometer.
Cleanliness: Check whether the inside of the refrigerator is clean, whether there are residual stains or expired samples.
Power supply and stability: Ensure stable power supply voltage and avoid frequent power outages; Ultra low temperature refrigerators need to be equipped with delay protectors.
3. Pre cooling (new refrigerator or unused for a long time)
Newly purchased or restarted refrigerators after power failure should be pre cooled to the set temperature with no load before placing samples.
Avoid placing too many samples at once to prevent the compressor from running under overload.
2、 Proper storage of samples in laboratory refrigerators:
1. Classified storage
Divided by temperature:
4 ℃ refrigerator: stores reagents, culture media, temporary samples, etc. for short-term storage.
-20 ℃ freezer: stores enzymes, proteins, nucleic acids, cell cryovials, etc. for long-term preservation.
-80 ℃ refrigerator: stores precious samples (such as cell lines, rare DNA/RNA), and long-term archived samples.
Partition by type:
Avoid cross contamination, such as storing chemical reagents separately from biological samples and sealing volatile reagents.
2. Reasonable placement
Leave ventilation space: Do not block the air outlet or fill the refrigerator, ensure smooth circulation of cold air.
Use storage tools:
Place small samples in a sealed box or freezer rack to avoid direct contact with the inner wall of the refrigerator.
Large volume samples (such as culture dishes, centrifuge tubes) are placed in layers on trays or shelves.
Clear labeling: All samples must be labeled with their name, concentration, date, and responsible person for easy identification and retrieval.
3. Avoid repeated freeze-thaw cycles
When taking samples, the speed should be fast to reduce the opening time of the refrigerator door.
Cherish frozen samples, plan the experimental dosage in advance, and avoid degradation caused by multiple thawing.
