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How to choose suitable pure water according to experimental needs?
Date: 2025-10-29Read: 0
As a commonly used reagent in laboratories, the quality of pure water is crucial, which can affect the accuracy, reliability, and reproducibility of experimental results, reduce the service life of precision analytical instruments, and increase maintenance costs. Pure water is not a single substance, but contains five categories of impurities: particles, ions, organic matter, microorganisms, and gases. Different experiments have different requirements for the content of impurities in water.
GB/T 6682-2008 "Specifications and Test Methods for Water Used in Analytical Laboratories" classifies laboratory water into three levels: first level water, second level water, and third level water.
Table 1: Classification and Main Specifications of Laboratory Water Used According to GB/T 6682-2008
如何根据实验需求选择合适的纯水?
  First grade water (ultrapure water):It is usually made by passing second (third) grade pure water through ion exchange (DI) resin and then through a 0.2 micron terminal filter. Mainly used for:
  instrumental analysisFor example, experiments such as HPLC, IC, AAS, ICP, UV Vis, LC-MS, ICP-MS, etc;
  Biomedical ExperimentFor example, experiments in molecular biology, cell biology, immunology, etc.
Lefeng's flagship modelGenie G, Genie PURIST Ultra Pure Water SystemBy integrating optimized flow path design, advanced purification technologies (such as continuous current deionization EDI and reverse osmosis), carefully selected formula fillers (LeFil and OrganeFil) for ultra purification columns, and IoT IoT technology, stable and reliable ultrapure water can be provided. collocationGenie De ion Purification Water Terminal for Trace AnalysisIt can prepare ultrapure water for analysis with ultra-low elemental levels, with ion impurity content as low as 0.1 ng/L or even 0.01 ng/L,Suitable for users who are concerned about "micro water quality" such as semiconductors and microelectronics.
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  Secondary water (EDI pure water):Produced through ion exchange or electrodeionization exchange (EDI) technology. Suitable for supporting water supply for routine analysis and experimental instruments, such as clinical biochemical analyzers, large high-pressure sterilization pots, etc; Preparation of buffer solution and culture medium.
  Super-Genie E500High flow intelligent pure water workstationBy reasonably configuring pure water storage, distribution, and transportation pipelines, stable EDI pure water can be provided to the water points of the entire building, which can supply water to fermentation tanks, chromatography systems, and other facilities of some biopharmaceutical companies,Meet the dual requirements of high water quality and large water volume.
如何根据实验需求选择合适的纯水?

  Third grade water (RO pure water):Produced through RO reverse osmosis technology. Mainly used for cleaning conventional vessels, preparing conventional reagents, and using water for commonly used experimental instruments such as water baths. The RO aquatic water flow rate that Lefeng can provide can reach up to 600L/h, and can supply up to 14000 liters of pure water per day for single or multiple laboratories.
currentlyConductivity (resistivity), TOC (total organic carbon), and microorganisms are the main indicators for evaluating the quality of pure waterFor experiments with strict water requirements, we not only focus on 18.2 M Ω· cm, but also pay attention to the content of individual ions in the water, such as trace metal ions such as Fe, Cu, Zn, Pb, As, Ni, etc; Trace inorganic anions such as Cl -, SO42-, NO3-, etc. Some fields, such as semiconductors and pharmaceuticals, have special requirements for water quality and also need to refer to specific industry standards such as ASTM and national pharmacopoeias.
Choosing the appropriate water quality is only the first step in ensuring the success of the experiment. To ensure the authenticity, reliability, and reproducibility of experimental data, it is also necessary to combine good maintenance and water usage habits. Based on years of experience in pure water application, Lefeng has summarized many usage and maintenance precautions. Please click on the following link to view more content, so that you can use water worry free!
  1)Principle of using ultrapure water: one center, two basic points
  2)Do you know these methods for obtaining ultrapure water?
  3)The maintenance of a pure water machine is very important, and regular "health checks" are key
  About Lefeng Biotechnology
Rephil Bioscience, Ltd. is a company under the Duoning Group that specializes in the research, design, and manufacturing of water purification and laboratory separation and purification products, serving users in the high-tech biotechnology and life science fields. Lefeng Company focuses on global development and has established sales offices in nearly 20 countries including China, the United States, France, India, and South Africa. It also provides OEM and ODM services to large international companies, and its products are sold to more than 100 countries including Europe, America, Japan, and South Korea. Many Ivy League universities, Harvard Medical School Dana Farber Cancer Institute, Johns Hopkins University School of Medicine, and University of Pennsylvania School of Medicine are users of Lefeng pure water equipment. Established for over ten years, LeFeng has continuously invested in research and development, creating its own product brand RephiLe and launching multiple new concept products such as the Genie series pure water system with wireless connectivity and the intelligent high flow pure water workstation Super Genie. At present, Lefeng provides a full range of ultra purification column series suitable for various applications, including low magnesium type, low boron type, ICP-MS special type, etc. It also offers compatible consumables for multiple Millipore pure water systems, allowing domestic brands to provide users with services comparable to imported products.