Standard Operating Procedures for the Use and Maintenance of CO2 Incubators

1 Purpose

establishCO₂The standard operating procedures for the use and maintenance of incubators ensure that operators use them correctly.

2 Scope

Applicable to CO₂Operation and maintenance of the incubator.

3 Definitions

CO₂A culture box is created by simulating the formation of a cell like structure inside the box/The growth environment of the organization inside the organism requires a stable temperature (37 ° C) and stable CO in the incubator₂Level（A device for in vitro culture of cells/tissues, with a constant pH value of 7.2-7.4 and a relatively high saturation humidity of 95%, is an advanced instrument for cell, tissue, and bacterial culture. It is a key equipment necessary for immunology, oncology, genetics, and bioengineering.

4 Responsibilities

Equipment users shall execute thisSOP。

5 Programs

5.1 Instrument Structure

Carbon dioxide cultivation is composed of a carbon dioxide cylinder and a casing.

5.2 Operating Procedures for Use

5.2.1 Preparation

(1) When the pressure of the carbon dioxide cylinder decreases, purchase in advance and prepare spare parts.

(2) Prepare 2-3L of sterile water or saturated copper sulfate solution every two weeks.

5.2.2 Operation

(1) Confirm that the instrument and equipment are in the correct installation state, and that the power supply, carbon dioxide gas, and other connections are correct. Open CO₂Gas supply valve; AttentionCO₂The gas supply must be output from the pressure reducing valve and maintained at a pressure of0.5～1bar， Cannot exceed 1 bar.

(2) Start the program

Before starting, turn on the power switch and set the working parameters. (Note: The pressure sensing switch of the newly opened incubator can only work normally after 2-3 hours of power on!).

(3) Run the program, set the working parameters, and follow the required gas values and temperatures for the experiment, such as 37 ℃ and 5% CO₂After stabilization, the sample can be placed.

(4) Run the setup program.

5.3 Cleaning Operating Procedures

5.3.1 Wipe the stainless steel partition and inner container inside the incubator with 75% alcohol every month, and irradiate with a handheld ultraviolet lamp for 1 hour.

5.4 Maintenance Operation Procedures

5.4.1 The surface of the machine should be cleaned regularly and kept clean.

5.4.2 When not in use, close the program, turn off the gas cylinder, and turn off the power. When not in use for a long time, the power plug should be unplugged.

5.4.3 Cleaning/Sterilization of Boxes and Accessories (once a month).

5.4.4 Wipe all accessories with purified water, wipe dry with non-woven fabric or paper, and then spray with 75% alcohol for disinfection before wiping dry. Ensure that the corners and top of the inner container have been treated similarly, and then use a handheld UV lamp to irradiate for one hour.

5.4.5 Change water or saturated copper sulfate solution in the humidification tray (once every 2 weeks).

5.5 Fill in Records

Fill in promptlyCO₂Incubator temperatureCO₂Concentration record.

5.6 Precautions

5.6.1 When the incubator is not in use for a long time, the power plug should be unplugged, the water or saturated copper sulfate solution in the tray should be poured out, the chamber should be cleaned, and the door should be closed after drying.

5.6.2 When picking up and delivering culture materials, it is advisable to avoid opening the door too wide and frequently opening and closing the incubator door to prevent CO emissions₂There are significant fluctuations in concentration, temperature, and relative humidity.