Serum-free culture mediumAnd reagents are widely used to cultivate mammalian and spineless animal cells for the preparation of monoclonal antibodies, viral antigens, and recombinant proteins. Most serum-free products contain transferrin, which transports ions into cells, insulin, which regulates glucose uptake, as well as proteins such as albumin, fibronectin, and fetoprotein. These proteins play various functions in cell culture, such as adsorbing toxic compounds, resisting bioreactor shear forces, providing the matrix required for cell adhesion, and serving as carriers for lipids and other growth factors.

LONZA company serum-free culture medium
Type:
① Product Name:
UltraCUTURETM culture medium
Product Code:
12-725F UltraCULTURE MEDIUM(Serum-free general purpose medium) 500ml
Product Introduction: A universal serum-free culture medium
Scope of application: Cultivation of adherent and suspended mammalian cells, preparation of viral particles in vaccine production
② Product Name:
PC-1TM culture medium
产品货号: 77232 PC-1Complete Serum-free Medium 500ml
77230 PC-1 Complete Serum-free Medium(powder plus supplement) 10L
344022 PC-1 Supplement (50) 10ml
Product Introduction: It is a low protein, serum-free culture medium. PC-1 medium is designed for various research and industrial applications, using a medium formulated with limited ingredients to ensure that cells achieve optimal growth while maintaining the lowest protein content.
Scope of application: Cultivate primary cells and adherent dependent cells
③ Product Name:
UltraMEM Reduced Serum medium
Product Code:
12-743F UltraMEM Reduced Serum Medium
(Low-protein medium containing L-glutamine &ITES) 500ml
12-745F UltraMEM Reduced Serum Medium
(Protein-free basal medium without L-glutamine) 500ml
Product Introduction: A limited culture medium used to support the normal growth of various adherent reliable cells at low serum concentrations.
Scope of application: Cultivate adherent reliable cells
④ Product Name:
UltraCHOTM medium
产品货号: 12-724Q UltraCHO Meidum(1*liquid with L-glutamine) 1L
12-715F UltraCHO Meidum(1*liquid without nucleosides) 500mL
17-811A UltraCHO Supplement(Non-sterile) 100ml
Product Introduction: The culture medium is suitable for cultivating transfected and untransfected Chinese hamster ovary (CHO) cells, expressing heavy sister protein.
Scope of application: Cultivate CHO cells; Synthesis of recombinant proteins.
⑤ Product Name:
UltraMDCKTM medium
Product Code: 12-749Q UltraMDCK Meida 1L
Product Introduction: It is a serum-free limited culture medium suitable for high/low plate density cultivation of MADIN-DABBY canine kidney cells (MDCK)
Scope of application: Cultivate MDCK cells for in vitro diagnostics (IVD)
⑥ Product Name:
Pro293TMCDM system
Product Code:
12-764Q PRO 293A-CDM 1L
12-765Q PRO 293S-CDM 1L
Product Introduction: The Pro293 limited medium system is developed specifically for promoting the synthesis and downstream reactions of recombinant proteins in 293 cells (transformed human embryonic kidney cells). These formulas do not require any animal derived ingredients to support high-density cultivation
Scope of application: 293 cell recombinant protein and synthesis
⑦ Product Name:
Insect XPRESTM culture medium
Product Code: 12-730Q Insect XPRESS Medium 1L
Product Introduction: Adopting a protein free formula, suitable for cultivating insect cell lines derived from Spodoptera frugiperda (Sf9 and Sf21).
Scope of application: Proliferation of rod-shaped viruses and expression of recombinant proteins.
⑧ Product Name:
HL-1TM * serum-free culture medium
产品货号: 77203 HL-1 Complete Serum-free Medium(powder) 10L
Product introduction: HL-1TM * serum-free medium is a limited medium with a protein content of less than 30ug/ml. HL-1TM * serum-free medium does not contain bovine serum albumin and other unknown protein mixtures.
Scope of application: Serum free culture of differentiated cells derived from hybridoma cells and lymphocytes.
⑨ Product Name:
UltraDOMATM culture medium
Product Code: 12-723 BULtraDOMA Medium 500ml
Product Introduction: Suitable for the cultivation of medium, mouse, and chimeric hybridoma cells in hollow fiber reactors, as well as batch cultivation of the aforementioned cells.
Scope of application: Cultivation of hybridoma cells, preparation of monoclonal antibodies.
⑩ Product Name:
UltraDOMA-PFProtein free culture medium
Product Code: 12-727F FUltraDOMA-P.F. Protein free Medium 500ml
08-024B Ultra DOMA-P.F.Protein-free Medium 10L
Product Introduction: A protein free culture medium suitable for the cultivation of hybridoma cell lines derived from mice, humans, and chimeric cells.
Scope of application: Cultivation of hybridoma cells and myeloma cells, preparation of monoclonal antibodies.
11.
X-VIVOculture medium
Product Name: X-VIVO 10
Product Code:
04-380Q X-VIVO 10 with Gentamicin and Phenol Red, 1 L
04-743Q X-VIVO 10 w/o Gentamicin and Phenol Red, 1 L
Product Introduction: Designed to facilitate the preparation of lymphokine activated killer cells (LAK) in a serum-free environment.
Product Name: X-VIVO 15
Product Code:
04-418Q X-VIVO 15 with Gentamicin and Phenol Red, 1 L
04-744Q X-VIVO 15 w/o Gentamicin or Phenol Red, 1 L
Product Introduction: It is similar in composition to X-VIVO 10 and has been adjusted and optimized to be suitable for the proliferation of tumor infiltrating lymphocytes (TIL) under serum-free conditions. Its formula is helpful for the proliferation of CD3+cells isolated and purified from peripheral and human tumors. It is also used to maintain the growth of human monocytes, macrophages, cell lines, granulocytes, and natural killer cells (NK).
Product Name: X-VIVO 20
产品货号: 04-448Q X-VIVO 20 with Gentamicin and Phenol Red, 1 L
Product Introduction: LAK cells are prepared from high-density peripheral lymphocytes that have been improved and optimized to facilitate monocyte depletion.
Price: To be determined
Storage conditions: 2 ℃ -8 ℃
The application of three types of cultured partial cell types is as follows:
Human and mouse lymphokine activated killer cells (LAK); Peripheral blood lymphocytes (PBL); Tumor infiltrating lymphocytes (TIL) in humans and mice; Human T cells; Human and mouse macrophages; Human and mouse hematopoietic stem cells (clone formation and proliferation); Cryopreservation of human tissues.
Other applications of X-VIVO products include:
Proliferation of PBL; TIL proliferation; Cryopreservation and transplantation of organs; Cultivation of human monocytes and macrophages; Cultivation of stem cells; Cultivation of dendritic cells.
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