Analyze solutions-Ethanol content in bloodThe headspace gas chromatography method

Analysis purpose and principle:

This solution uses HS GC-FID to measure the ethanol content in the blood; Using tert butanol as the internal standard, a splitter is used to perform dual column parallel connection. After the sample is split, it enters FID1 and FID2 respectively. The different polarities of the dual columns are used to more accurately qualitatively and quantitatively analyze the progress of ethanol residue.

Experimental instruments:

HS G51Pro headspace gas chromatograph

N2 steel cylinder 40L purity>99.999%

Air: Oil free air generator (XGA-380A)

Hydrogen: High purity hydrogen generator (XGH-300E)

Analysis conditions:

Headspace conditions: Furnace temperature: 70 ℃, transmission tube: 130 ℃, valve body: 110 ℃

Balance time: 40min, pressurization time: 12s, sampling time: 12s, ring leveling time: 10s, injection time: 12s, blowback time: 5min

Conclusion:

Under these analysis conditions, the time RSD is less than 0.5%, the area RSD is less than 1.5%, the reproducibility is good, the sensitivity is high, and the linearity is good. The detection results are accurate and reliable. The baseline noise is 2-3UV, meeting the minimum detection limit requirement. A dual column system is used, which avoids the possibility of misjudgment due to different compounds exhibiting the same retention time on a chromatographic column with the same properties. This method is simple to operate, has high sensitivity, low detection limit, and strong applicability.