Automatic column passing machine is an efficient and automated chromatographic separation equipment based on column chromatography technology, mainly used for sample separation and purification in chemical synthesis, natural product extraction, drug purification and other processes, which can improve the efficiency and accuracy of separation and purification.

The working principle of the automatic column passing machine is based on column chromatography technology. First, load the mixture sample into the column, which is filled with one or more adsorbents. Different compounds in the sample have different adsorption capacities on the adsorbents. When the sample passes through the column, the composition or concentration of the mobile phase gradually changes, and different compounds will move at different rates in the column, thereby achieving separation in the column. Then, the compounds adsorbed on the column are eluted, and the eluted compounds will flow out of the column in a separated form and be collected to obtain the purified target compound.

Advantage:

Efficiency: The separation efficiency of a single chromatographic column is 3-5 times that of manual operation. For example, in the separation of traditional Chinese medicine compound extracts, monomer compound purification can be quickly completed. Supports continuous operation without the need for manual monitoring.

Accuracy: The pressure control accuracy reaches ± 0.1MPa, avoiding column pressure fluctuations caused by manual operation, protecting the chromatographic column and extending its service life. Using high-precision gradient elution, impurities with similar polarity can be separated.

Automation and Safety: Integrated fraction collector that can automatically switch collection bottles based on peak area, reducing human error.

Data Traceability: Automatically saves elution programs and chromatograms, meets GMP requirements for data traceability, and is suitable for bridging scenarios from laboratory to pilot production in drug development.